The nonmuscle myosin regulatory light chain gene mlc-4 is required for cytokinesis, anterior-posterior polarity, and body morphology during Caenorhabditis elegans embryogenesis.
The nonmuscle myosin regulatory light chain gene mlc-4 is required for cytokinesis, anterior-posterior polarity, and body morphology during Caenorhabditis elegans embryogenesis.
复制标题
DOI：
10.1083/jcb.146.2.439
复制
发表时间：
1999-07-26
期刊：
影响因子：
--
通讯作者：
Bowerman B
中科院分区：
文献类型：
--
作者：
Shelton CA;Carter JC;Ellis GC;Bowerman B
利用RNA介导的遗传干扰在表型筛选中，我们在秀丽隐杆线虫中发现了一个保守的非肌肉肌球蛋白II调节轻链基因，我们将其命名为MLC-4。母体提供的MLC-4功能是减数分裂和有丝分裂期间的细胞质分裂以及受精后建立前后不对称(a-p)所必需的。降低非肌肉肌球蛋白II基因MLC-4或nMy-2的功能也会导致线虫受精卵中极化细胞质流动的丧失，这支持了可能需要细胞质流动来建立a-p差异的模型。在这些胚胎中，胚系P颗粒在细胞质流动时的定位也丢失了，尽管P颗粒在第一次有丝分裂后确实定位于后极。这一结果表明，细胞质流动或MLC-4/nMy-2活性以外的机制可以在线虫受精卵中产生一些a-p不对称。通过分离缺失等位基因，我们证明了去除合子MLC-4功能会导致胚胎发育过程中的伸长表型。MLC-4/绿色荧光蛋白转基因在侧行的皮下细胞中表达，这些细胞在MLC-4突变动物的伸长过程中不能正确地改变形状。
Using RNA-mediated genetic interference in a phenotypic screen, we identified a conserved nonmuscle myosin II regulatory light chain gene in Caenorhabditis elegans, which we name mlc-4. Maternally supplied mlc-4 function is required for cytokinesis during both meiosis and mitosis and for establishment of anterior-posterior (a-p) asymmetries after fertilization. Reducing the function of mlc-4 or nmy-2, a nonmuscle myosin II gene, also leads to a loss of polarized cytoplasmic flow in the C. elegans zygote, supporting models in which cytoplasmic flow may be required to establish a-p differences. Germline P granule localization at the time of cytoplasmic flow is also lost in these embryos, although P granules do become localized to the posterior pole after the first mitosis. This result suggests that a mechanism other than cytoplasmic flow or mlc-4/nmy-2 activity can generate some a-p asymmetries in the C. elegans zygote. By isolating a deletion allele, we show that removing zygotic mlc-4 function results in an elongation phenotype during embryogenesis. An mlc-4/green fluorescent protein transgene is expressed in lateral rows of hypodermal cells and these cells fail to properly change shape in mlc-4 mutant animals during elongation.
登录
查看更多内容
影响因子：
4.8
作者：
Kureishi, Y;Kobayashi, S;Ito, M
通讯作者：
Ito, M
影响因子：
56.9
作者：
CHALFIE, M;TU, Y;PRASHER, DC
通讯作者：
PRASHER, DC
影响因子：
2.7
作者：
KIRBY, C;KUSCH, M;KEMPHUES, K
通讯作者：
KEMPHUES, K
影响因子：
7.5
作者：
Bresnick, AR
通讯作者：
Bresnick, AR
影响因子：
64.8
作者：
Guo, S;Kemphues, KJ
通讯作者：
Kemphues, KJ