Most human proteins made in both nucleus and cytoplasm turn over within minutes.
Most human proteins made in both nucleus and cytoplasm turn over within minutes.
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DOI：
10.1371/journal.pone.0099346
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Cook PR
中科院分区：
文献类型：
--
作者：
Baboo S;Bhushan B;Jiang H;Grovenor CR;Pierre P;Davis BG;Cook PR
在细菌中，蛋白质合成可以与转录结合在一起，但在真核生物中，蛋白质合成被认为只发生在细胞质中。使用短至5 S的脉冲，我们发现三个类似物--L-叠氮高丙氨酸、嘌呤霉素(用‘点击’化学或免疫标记结合荧光后检测到)和‘Heavy’15N和13C标记的氨基酸(用二次离子质谱仪检测)--以对翻译抑制物敏感的过程进入细胞核和细胞质。核掺入占总多肽的很大比例，两个隔间的标记半衰期都不到一分钟；结果与大多数新制造的多肽在制造后不久就被销毁的结果一致。由于带有提前终止密码子的新生RNA(通过荧光原位杂交检测到)也被一种对翻译抑制物敏感的机制消除，多肽的核周转可能是校对终止密码子的RNA的副产品(这一过程被称为无义介导的衰变)。我们推测，这个先前隐藏的(‘暗物质’)多肽世界的明显浪费的周转与调节蛋白质的生产有关。
In bacteria, protein synthesis can be coupled to transcription, but in eukaryotes it is believed to occur solely in the cytoplasm. Using pulses as short as 5 s, we find that three analogues – L-azidohomoalanine, puromycin (detected after attaching fluors using ‘click’ chemistry or immuno-labeling), and amino acids tagged with ‘heavy’ 15N and 13C (detected using secondary ion mass spectrometry) – are incorporated into the nucleus and cytoplasm in a process sensitive to translational inhibitors. The nuclear incorporation represents a significant fraction of the total, and labels in both compartments have half-lives of less than a minute; results are consistent with most newly-made peptides being destroyed soon after they are made. As nascent RNA bearing a premature termination codon (detected by fluorescence in situ hybridization) is also eliminated by a mechanism sensitive to a translational inhibitor, the nuclear turnover of peptides is probably a by-product of proof-reading the RNA for stop codons (a process known as nonsense-mediated decay). We speculate that the apparently-wasteful turnover of this previously-hidden (‘dark-matter’) world of peptide is involved in regulating protein production.
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影响因子：
8
作者：
Dolan, Brian P.;Bennink, Jack R.;Yewdell, Jonathan W.
通讯作者：
Yewdell, Jonathan W.
影响因子：
3.7
作者：
Kapranov P;St Laurent G
通讯作者：
St Laurent G
影响因子：
5.6
作者：
Hansen, JL;Moore, PB;Steitz, TA
通讯作者：
Steitz, TA
DOI：
10.1126/science.1169237
发表时间：
2009-05-15
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Goldman SR;Ebright RH;Nickels BE
通讯作者：
Nickels BE
DOI：
10.1074/mcp.m111.011429
发表时间：
2012-03
期刊：
Molecular & cellular proteomics : MCP
影响因子：
--
作者：
Boisvert FM;Ahmad Y;Gierliński M;Charrière F;Lamont D;Scott M;Barton G;Lamond AI
通讯作者：
Lamond AI