Immunocytochemical Localization of Choline Acetyltransferase in the Microbat Visual Cortex.
Immunocytochemical Localization of Choline Acetyltransferase in the Microbat Visual Cortex.
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DOI：
10.1267/ahc.18018
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发表时间：
2018-10-31
影响因子：
2.4
通讯作者：
Jeon CJ
中科院分区：
文献类型：
--
作者：
Kim GH;Kim HG;Jeon CJ
本研究的目的是用标准免疫细胞化学和共聚焦显微镜观察微蝙蝠视皮层胆碱乙酰转移酶(ChAT)免疫反应(IR)纤维的组织结构。ChAT-IR纤维分布于视皮层各层，以第III层密度最高，第I层密度最低，但在视皮层内未见ChAT-IR细胞。ChAT-IR纤维分为两类：大、小曲张纤维。以前在哺乳动物的视皮层、斜角带核、无名质和大细胞基底核中发现的胆碱能纤维的来源，在小蝙蝠中都含有强标记的ChAT-IR细胞。斜角带核、无名核和大细胞基底核ChAT-IR细胞的平均直径分别为16.12μm、13.37μm和13.90μm。我们用ChAT和γ-氨基丁酸(GABA)进行的双重标记，以及用ChAT、GABA和突触后密度95(PSD-95)进行的三次标记，表明一些ChAT-IR纤维与小蝙蝠视觉皮质中的GABA能细胞接触。我们的结果应该能更好地理解夜间蝙蝠的视觉系统。
The purpose of the present study was to investigate the organization of choline acetyltransferase (ChAT)-immunoreactive (IR) fibers in the visual cortex of the microbat, using standard immunocytochemistry and confocal microscopy. ChAT-IR fibers were distributed throughout all layers of the visual cortex, with the highest density in layer III and the lowest density in layer I. However, no ChAT-IR cells were found in the microbat visual cortex. ChAT-IR fibers were classified into two types: small and large varicose fibers. Previously identified sources of cholinergic fibers in the mammalian visual cortex, the nucleus of the diagonal band, the substantia innominata, and the nucleus basalis magnocellularis, all contained strongly labeled ChAT-IR cells in the microbat. The average diameter of ChAT-IR cells in the nucleus of the diagonal band, the substantia innominata, and the nucleus basalis magnocellularis was 16.12 μm, 13.37 μm, and 13.90 μm, respectively. Our double-labeling study with ChAT and gamma-aminobutyric acid (GABA), and triple labeling with ChAT, GABA, and post synaptic density 95 (PSD-95), suggest that some ChAT-IR fibers make contact with GABAergic cells in the microbat visual cortex. Our results should provide a better understanding of the nocturnal bat visual system.
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影响因子：
3.3
作者：
ECKENSTEIN, FP;BAUGHMAN, RW;QUINN, J
通讯作者：
QUINN, J
影响因子：
11.2
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ALEXIANU, ME;HO, BK;APPEL, SH
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1.3
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Pessoa, Daniel M. A.
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通讯作者：
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