Rapid evolution of pyrethroid resistance prevalence in Anopheles gambiae populations from the cities of Douala and Yaoundé (Cameroon).
Rapid evolution of pyrethroid resistance prevalence in Anopheles gambiae populations from the cities of Douala and Yaoundé (Cameroon).
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DOI：
10.1186/s12936-015-0675-6
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发表时间：
2015-04-14
期刊：
影响因子：
3
通讯作者：
Wondji CS
中科院分区：
文献类型：
--
作者：
Antonio-Nkondjio C;Tene Fossog B;Kopya E;Poumachu Y;Menze Djantio B;Ndo C;Tchuinkam T;Awono-Ambene P;Wondji CS
疟疾病媒对城市地区的适应正成为疟疾控制的一项严重挑战。本研究介绍了2010 - 2013年杜阿拉市和雅温德瓦尔市蚊子种群中拟除虫菊酯类杀虫剂耐药性的演变情况。对从田间收集的冈比亚按蚊幼虫饲养的2至4龄未饲喂的冈比亚按蚊成虫进行了氯菊酯和溴氰菊酯敏感性试验。采用TaqMan法筛选对氯菊酯和溴氰菊酯产生抗性和对照的蚊虫，检测kdr等位基因的存在。蚊子属于安蚊。冈比亚菌复合体采用PCR方法进行物种和分子形态鉴定。对来自不同孳生地的蚊子含有电压门控钠通道内含子-1上游的基因组区域进行了测序和比较。在杜阿拉市采集的冈比亚按蚊标本均为科鲁兹按蚊。在雅芳，安。冈比亚和安哥拉。记录了Coluzzii。2010 - 2013年，两地蚊虫对氯菊酯和溴氰菊酯的死亡率迅速下降。氯菊酯和溴氰菊酯的死亡率分别为80.3 ~ 22.3%和94.4 ~ 59.7%。kdr等位基因L1014F和L1014S均有记录。kdr等位基因的频率在研究期间迅速增加，雅温顿地区的频率从44%到88.9%不等，杜阿拉地区的频率从68%到81%不等。对电压门控钠通道的1,228 bp的插入-1区域的测序显示存在五种不同的单倍型。这些单倍型在安省被大量记录。coluzzii样本。使用Fu 's和Tajima f统计数据未发现来自不同繁殖栖息地的样品中最近选择性扫描内含子-1序列的证据。本研究支持来自Douala和yaound<s:1>市的病媒种群对拟除虫菊酯类杀虫剂的抗性快速进化，并呼吁立即采取行动，防治对拟除虫菊酯类杀虫剂抗性蚊子的日益流行。
The adaptation of malaria vectors to urban areas is becoming a serious challenge for malaria control. The study presents the evolution of pyrethroid resistance in mosquito populations from the cities of Douala and Yaoundé between 2010 and 2013. Susceptibility tests to permethrin and deltamethrin were carried out with two- to four-day old unfed Anopheles gambiae sensu lato adults raised from larvae collected from the field. Mosquitoes resistant to permethrin and deltamethrin and control were screened to detect the presence of the kdr alleles using the TaqMan assays. Mosquitoes belonging to the An. gambiae complex were subjected to PCR assays designed for species and molecular forms identifications. The genomic region containing the upstream of intron-1 of the voltage-gated sodium channel was sequenced and compared between mosquitoes originating from different breeding habitats. Anopheles gambiae s.l. specimens collected from the city of Douala were all Anopheles coluzzii. In Yaoundé, both An. gambiae and An. coluzzii were recorded. A rapid decrease of mosquito mortality to permethrin and deltamethrin was recorded between 2010 and 2013 in the two cities. The mortality rate varied from 80.3 to 22.3% and 94.4 to 59.7% for permethrin and deltamethrin, respectively. Both kdr alleles L1014F and L1014S were recorded. The frequency of kdr alleles increased rapidly over the study period, varying from 44 to 88.9% in Yaoundé and from 68 to 81% in Douala. The sequencing of a 1,228 bp region of intro-1 of the voltage-gated sodium channel revealed the presence of five different haplotypes. A high number of these haplotypes were recorded in An. coluzzii samples. No evidence for a recent selective sweep on intron-1 sequence within samples originating from different breeding habitat was detected using Fu’s and Tajima Fs statistics. The present study supports rapid evolution of pyrethroid resistance in vector populations from the cities of Douala and Yaoundé and calls for immediate action to fight against the increasing prevalence of pyrethroid-resistant mosquitoes.
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影响因子：
2.6
作者：
Fanello, C;Petrarca, V;Coluzzi, M
通讯作者：
Coluzzi, M
影响因子：
3.3
作者：
Moreno, M.;Vicente, J. L.;Benito, A.
通讯作者：
Benito, A.
影响因子：
3
作者：
Antonio-Nkondjio C;Youmsi-Goupeyou M;Kopya E;Tene-Fossog B;Njiokou F;Costantini C;Awono-Ambene P
通讯作者：
Awono-Ambene P
影响因子：
3.2
作者：
Nwane P;Etang J;Chouaїbou M;Toto JC;Koffi A;Mimpfoundi R;Simard F
通讯作者：
Simard F
影响因子：
3
作者：
Bass, Chris;Nikou, Dimitra;Donnelly, Martin J.;Williamson, Martin S.;Ranson, Hilary;Ball, Amanda;Vontas, John;Field, Linda M.
通讯作者：
Field, Linda M.