Gold nanorods inhibit respiratory syncytial virus by stimulating the innate immune response.
Gold nanorods inhibit respiratory syncytial virus by stimulating the innate immune response.
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DOI：
10.1016/j.nano.2016.06.006
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发表时间：
2016-11
期刊：
影响因子：
--
通讯作者：
Singh SR
中科院分区：
文献类型：
--
作者：
Bawage SS;Tiwari PM;Singh A;Dixit S;Pillai SR;Dennis VA;Singh SR
呼吸道合胞病毒（RSV）可导致婴儿、儿童和老年人严重肺炎和细支气管炎。正在探索使用金属纳米颗粒作为潜在的治疗剂来对抗呼吸道病毒，如流感病毒、副流感病毒和腺病毒。在这项研究中，我们发现金纳米棒（GNRs）抑制RSV在HEp-2细胞和BALB/c小鼠分别为82%和56%。RSV抑制与由于GNR介导的TLR、NOD样受体和RIG-I样受体信号传导途径引起的抗病毒基因显著上调相关。肺的透射电子显微镜检查显示内吞囊泡中存在GNR，组织学分析表明与RSV清除相关的中性粒细胞、嗜酸性粒细胞和单核细胞浸润。此外，肺中细胞因子和趋化因子的产生表明免疫细胞的募集以对抗RSV复制。据我们所知，这是第一个在体外和体内的报告，提供了可能的抗病毒机制GNRs对RSV。金纳米棒（GNRs）在体外和体内抑制呼吸道合胞病毒（RSV）。抗病毒基因表达研究显示Toll样受体、NOD样受体和RIG-I样受体信号通路相互作用。透射电子显微镜、组织学研究和细胞因子分析表明，GNRs刺激先天免疫应答，导致RSV抑制。
Respiratory syncytial virus (RSV) causes severe pneumonia and bronchiolitis in infants, children and older adults. The use of metallic nanoparticles as potential therapeutics is being explored against respiratory viruses like Influenza, Parainfluenza and Adenovirus. In this study, we showed that gold nanorods (GNRs) inhibit RSV in HEp-2 cells and BALB/c mice by 82% and 56%, respectively. The RSV inhibition correlated with marked upregulated antiviral genes due to GNR mediated TLR, NOD-like receptor and RIG-I-like receptor signaling pathways. Transmission electron microscopy of lungs showed GNRs in the endocytotic vesicles and histological analyses indicated infiltration by neutrophils, eosinophils and monocytes correlating with clearance of RSV. In addition, production of cytokines and chemokines in the lungs indicate recruitment of immune cells to counter RSV replication. To our knowledge, this is the first in vitro and in vivo report that provides possible antiviral mechanisms of GNRs against RSV. Gold nanorods (GNRs) inhibit respiratory syncytial virus (RSV) in vitro and in vivo. The antiviral gene expression study showed interplay of Toll-like receptor, NOD-like receptor and RIG-I-like receptor signaling pathways. Transmission electron microcopy, histological investigation and cytokine analysis indicate that GNRs stimulates innate immune response resulting in RSV inhibition.
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