Renalase and its receptor, PMCA4b, are expressed in the placenta throughout the human gestation.
Renalase and its receptor, PMCA4b, are expressed in the placenta throughout the human gestation.
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DOI：
10.1038/s41598-022-08817-6
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发表时间：
2022-03-23
影响因子：
4.6
通讯作者：
Konnikova L
中科院分区：
文献类型：
--
作者：
Wang M;Silva T;Toothaker JM;McCourt BT;Shugrue C;Desir G;Gorelick F;Konnikova L
胎盘的功能需要有组织的生长、营养物质的传递和母胎界面之间的抗炎环境，但对其功能至关重要的胎盘因素仍不清楚。肾酶是一种促进生存、抗炎的黄素蛋白，在其他组织中起着至关重要的作用。我们研究了肾素酶在胎盘发育中的潜在作用。应用聚合酶链式反应、批量RNA测序、免疫组织化学和免疫荧光技术对人中期胎盘和足月胎盘的蜕膜、胎盘绒毛和绒毛膜进行了肾素酶及其结合蛋白PMCA4b和PZP的免疫荧光检测。免疫组织化学定量检测肾酶在17周至足月的时间进程中的分布。在胎盘组织和器官中检测内源性肾氨酸酶的产生。肾氨酸酶及其受体PMCA4b转录本和蛋白在胎盘各层均有表达。在蜕膜样本中，估计的RNLS蛋白水平不随妊娠变化。然而，胎盘绒毛中含有的肾酶免疫反应细胞在胎儿中比足月胎盘更多。RNLS与胎盘绒毛内Hofbauer细胞和滋养层细胞的标记物共同标记。滋养层细胞内源性产生RNLS、PMCA4b和PZP在胎盘类器官中得到证实。肾氨酸酶在胎盘组织中广泛表达，尤其在Hofbauer细胞和滋养层细胞中表达，提示肾氨酸酶在胎盘发育和功能中具有潜在的作用。未来的研究应该评估肾酶在正常和患病的人胎盘中的作用。
Placental function requires organized growth, transmission of nutrients, and an anti-inflammatory milieu between the maternal and fetal interface, but placental factors important for its function remain unclear. Renalase is a pro-survival, anti-inflammatory flavoprotein found to be critical in other tissues. We examined the potential role of renalase in placental development. PCR, bulk RNA sequencing, immunohistochemistry, and immunofluorescence for renalase and its binding partners, PMCA4b and PZP, were performed on human placental tissue from second-trimester and full-term placentas separated into decidua, placental villi and chorionic plates. Quantification of immunohistochemistry was used to localize renalase across time course from 17 weeks to term. Endogenous production of renalase was examined in placental tissue and organoids. Renalase and its receptor PMCA4b transcripts and proteins were present in all layers of the placenta. Estimated RNLS protein levels did not change with gestation in the decidual samples. However, placental villi contained more renalase immunoreactive cells in fetal than full-term placental samples. RNLS co-labeled with markers for Hofbauer cells and trophoblasts within the placental villi. Endogenous production of RNLS, PMCA4b, and PZP by trophoblasts was validated in placental organoids. Renalase is endogenously expressed throughout placental tissue and specifically within Hofbauer cells and trophoblasts, suggesting a potential role for renalase in placental development and function. Future studies should assess renalase’s role in normal and diseased human placenta.
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影响因子：
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作者：
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通讯作者：
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DOI：
10.1080/14767058.2016.1186637
发表时间：
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影响因子：
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作者：
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影响因子：
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作者：
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