Using next-generation sequencing to detect oral microbiome change following periodontal interventions: A systematic review.
Using next-generation sequencing to detect oral microbiome change following periodontal interventions: A systematic review.
复制标题
使用下一代测序检测牙周干预后口腔微生物组的变化：系统评价
DOI：
10.1111/odi.13405
复制
发表时间：
2021-07
期刊：
影响因子：
3.8
通讯作者：
Dai R
中科院分区：
文献类型：
--
作者：
Zhang Y;Qi Y;Lo ECM;McGrath C;Mei ML;Dai R
本系统性综述旨在评价系统健康受试者牙周干预后基于下一代测序（NGS）-宏基因组分析的口腔微生物组变化。以“宏基因组学”和“口腔疾病”为检索词，采用结构化检索策略，检索PubMed、EMBASE和Web of Science，以确定有效的论文。纳入的研究是以英文发表的原始研究，使用宏基因组学方法分析牙周干预对牙周炎患者口腔微生物组的有效性。共纳入12篇文献。由于所选研究的异质性，未进行定量分析。关于α多样性在干预后如何变化的研究结果在各研究中并不一致。六项研究表明，使用主坐标/成分分析，干预前后收集的牙菌斑样本之间的微生物组成有明显的分离。干预前最常见的属是卟啉单胞菌、密螺旋体、坦纳菌和普雷沃菌，而干预后链球菌和放线菌通常增加并成为优势属。相关网络分析显示，干预后，网络的拓扑结构与相应的干预前样本相比有所不同。宏基因组研究的现有证据描述了牙周干预后口腔微生物组的复杂变化。
This systematic review was to evaluate the change of oral microbiome based on next‐generation sequencing (NGS)‐metagenomic analysis following periodontal interventions among systematically healthy subjects. A structured search strategy consisting of “metagenomics” and “oral diseases” was applied to PubMed, EMBASE, and Web of Science to identify effective papers. The included studies were original studies published in English, using metagenomic approach to analyze the effectiveness of periodontal intervention on oral microbiome among systematically healthy human subjects with periodontitis. A total of 12 papers were included in this review. Due to the heterogeneity of selected study, quantitative analysis was not performed. The findings as to how alpha diversity changed after interventions were not consistent across studies. Six studies illustrated clear separation of microbial composition between dental plaque samples collected before and after intervention using principal coordinates/component analysis. The most commonly detected genera before intervention were Porphyromonas, Treponema, Tannerella, and Prevotella, while Streptococcus and Actinomyces usually increased and became the dominant genera after intervention. Correlation network analysis revealed that after intervention, the topology of network was different compared to the corresponding pre‐interventional samples. Existing evidence of metagenomic studies depicts a complex change in oral microbiome after periodontal intervention.
登录
查看更多内容
影响因子：
3.7
作者：
Jünemann S;Prior K;Szczepanowski R;Harks I;Ehmke B;Goesmann A;Stoye J;Harmsen D
通讯作者：
Harmsen D
影响因子：
18.6
作者：
Paster, Bruce J.;Olsen, Ingar;Dewhirst, Floyd E.
通讯作者：
Dewhirst, Floyd E.
影响因子：
4.4
作者：
Hasturk, Hatice;Kantarci, Alpdogan;Van Dyke, Thomas E.
通讯作者：
Van Dyke, Thomas E.
影响因子：
9.4
作者：
Kumar, Purnima S.;Leys, Eugene J.;Griffen, Ann L.
通讯作者：
Griffen, Ann L.
DOI：
10.2174/1874210601206010255
发表时间：
2012
期刊：
The open dentistry journal
影响因子：
--
作者：
Laksmana T;Kittichotirat W;Huang Y;Chen W;Jorgensen M;Bumgarner R;Chen C
通讯作者：
Chen C