Determinants of plasma free acetaldehyde levels during the oxidation of ethanol: effects of chronic ethanol feeding.
Determinants of plasma free acetaldehyde levels during the oxidation of ethanol: effects of chronic ethanol feeding.
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乙醇氧化过程中血浆游离乙醛水平的决定因素：长期乙醇喂养的影响。
DOI：
10.1016/0006-2952(81)90168-4
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发表时间：
1981
影响因子：
5.8
通讯作者：
Lieber,CS
中科院分区：
文献类型：
--
作者：
Pikkarainen,PH;Gordon,ER;Lebsack,ME;Lieber,CS
通过一种改进的方法，在长期喂食乙醇的狒狒和在静脉注射乙醇期间成对喂食的对照组中测量血浆游离乙醛水平。在适当的负荷剂量后，以消除的速度注入乙醇，以达到三种不同血乙醇水平（50±10 mM， 10±2 mM或5±1 mM）之一的稳定状态。乙醛的产生速率是通过减去尿液和过期空气中损失的乙醇消除速率来计算的。在50 μM乙醛作为底物的手术活检样品中测量肝脏线粒体醛脱氢酶（AlDH）活性。慢性乙醇管理导致更高的血浆游离乙醛水平和更快的乙醛生产在每个水平的血液乙醇。当血液中乙醇浓度从5 mM增加到50 mM时，两组动物的血浆游离乙醛水平也升高。然而，乙醛的产生率只在酒精喂养的狒狒中增加。血浆游离乙醛与乙醛产率呈显著正相关（r= 0.69），与肝脏线粒体AIDH特异性活性呈显著负相关（r= - 0.59）。当这两个参数（乙醛产率/AIDH活性）结合在一起时，相关系数为0.84，这表明，除了产量增加外，分解代谢减少可能导致慢性消费后乙醛水平升高。
Plasma free acetaldehyde levels were measured by an improved method in baboons fed ethanol chronically and in pair-fed controls during an intravenous infusion of ethanol. After an appropriate loading dose, ethanol was infused at the rate of its elimination to achieve a steady state at one of three different blood ethanol levels (50 ± 10 mM, 10 ± 2 mM, or 5 ± 1 mM). The rate of production of acetaldehyde was calculated from the rate of ethanol elimination by subtracting losses into urine and expired air. Liver mitochondrial aldehyde dehydrogenase (AlDH) activity was measured in surgical biopsy samples with 50 μM acetaldehyde as substrate. Chronic ethanol administration resulted in both higher plasma free acetaldehyde levels and faster acetaldehyde production at each level of blood ethanol. When the blood level of ethanol was increased from 5 to 50 mM, the level of plasma free acetaldehyde also rose in both groups of animals. The rate of acetaldehyde production, however, increased only in alcohol-fed baboons. Plasma free acetaldehyde had a significant positive correlation with production rate of acetaldehyde (r= 0.69) and a significant negative correlation with liver mitochondrial AIDH specific activity (r= −0.59). When these two parameters were combined (acetaldehyde production rate/AIDH activity), a correlation coefficient of 0.84 resulted, suggesting that, in addition to increased production, decreased catabolism may contribute to the higher acetaldehyde levels seen after chronic consumption.
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DOI：
--
发表时间：
1968
期刊：
Biochemical and Biophysical Research Communications - BBRC
影响因子：
--
作者：
D. S. Beattie
通讯作者：
D. S. Beattie
狒狒酒精喂养和肝损伤的实验模型 1
DOI：
--
发表时间：
1974
期刊：
影响因子：
--
作者：
Charles S. Lieber;L. Decarli
通讯作者：
L. Decarli
血浆中“游离”乙醛的测定方法。
DOI：
--
发表时间：
1979
期刊：
Alcoholism: Clinical and Experimental Research
影响因子：
--
作者：
P. Pikkarainen;M. Salaspuro;C. Lieber
通讯作者：
C. Lieber
影响因子：
158.5
作者：
KORSTEN, MA;MATSUZAKI, S;LIEBER, CS
通讯作者：
LIEBER, CS
狒狒乙醇消除率的浓度依赖性：长期饮酒的影响。
DOI：
--
发表时间：
1980
期刊：
Alcoholism: Clinical and Experimental Research
影响因子：
--
作者：
P. Pikkarainen;C. Lieber
通讯作者：
C. Lieber