Stimulation of GLUT-1 glucose transporter expression in response to exposure to calcium ionophore A-23187.
Stimulation of GLUT-1 glucose transporter expression in response to exposure to calcium ionophore A-23187.
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暴露于钙离子载体 A-23187 后刺激 GLUT-1 葡萄糖转运蛋白表达。
DOI：
10.1152/ajpcell.1995.269.5.c1228
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发表时间：
1995
期刊：
影响因子：
--
通讯作者：
Ismail-Beigi,F
中科院分区：
文献类型：
--
作者：
Mitani,Y;Behrooz,A;Dubyak,GR;Ismail-Beigi,F
We tested the hypothesis that an increase in cytosolic calcium concentration stimulates glucose transporter isoform (GLUT-1) gene expression. Exposure of a rat liver cell line (Clone 9) to 3 microM A-23187 for 12 h resulted in 3-, 5-, and 10-fold increases in cytochalasin B-inhibitable 3-O-methyl-D-glucose transport, GLUT-1 protein, and GLUT-1 mRNA content, respectively. The induction of GLUT-1 mRNA in response to A-23187 is not preceded by a significant decrease in cell ATP content. This induction is prevented by 1,2-bis(2-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid in conjunction with ethylene glycol-bis(beta-aminoethyl ether)-N,N, N',N'-tetraacetic acid. To investigate the mechanism of GLUT-1 mRNA induction, we found that exposure to A-23187 stabilized GLUT-1 mRNA: with the employment of actinomycin D, GLUT-1 mRNA had a half-life of 1.5 and 5.5 h in control and A-23187-treated cells, respectively. In nuclear run-on assays, the rate of GLUT-1 gene transcription was stimulated 1.5- to 1.7-fold in nuclei isolated from cells exposed to A-23187 for either 30 min or 2 h. These results demonstrate that exposure to A-23187 stimulates GLUT-1 gene expression and that the increase in GLUT-1 mRNA content is mediated in part by enhanced GLUT-1 gene transcription as well as decreased GLUT-1 mRNA degradation. The increase in GLUT-1 mRNA content, in turn, is associated with increased cell GLUT-1 content and enhanced glucose transport.
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DOI：
10.1016/0167-4889(91)90090-k
发表时间：
1991-09-24
期刊：
BIOCHIMICA ET BIOPHYSICA ACTA
影响因子：
--
作者：
KURUVILLA, AK;PEREZ, C;LOEB, JN
通讯作者：
LOEB, JN
蛋白激酶 C 和离子转运抑制剂抑制原癌基因 c-fos 转录。
DOI：
10.1111/j.1432-1033.1987.tb10985.x
发表时间：
1987
期刊：
European journal of biochemistry
影响因子：
--
作者：
M. Shibanuma;T. Kuroki;K. Nose
通讯作者：
K. Nose
影响因子：
7.5
作者：
LEE A S
通讯作者：
LEE A S
影响因子：
18.2
作者：
J. Pessin;G. Bell
通讯作者：
J. Pessin;G. Bell
肥大细胞中白细胞介素 3 mRNA 表达的调节发生在转录后水平，并由钙离子介导。
DOI：
10.1073/pnas.87.2.777
发表时间：
1990
影响因子：
11.1
作者：
A. Wodnar‐Filipowicz;Christoph Moroni
通讯作者：
Christoph Moroni