Suppression of Urinary Voiding by Conditional High Frequency Stimulation of the Pelvic Nerve in Conscious Rats.
Suppression of Urinary Voiding by Conditional High Frequency Stimulation of the Pelvic Nerve in Conscious Rats.
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DOI：
10.3389/fphys.2018.00437
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发表时间：
2018
影响因子：
4
通讯作者：
Lovick TA
中科院分区：
文献类型：
--
作者：
Brouillard CBJ;Crook JJ;Irazoqui PP;Lovick TA
用仪器记录雌性Wistar大鼠膀胱压力并刺激左骨盆神经。将生理盐水（11.2 ml/h）通过t型管插入膀胱导管，连续输注膀胱，引起反复空腔。在每只实验动物（n = 6）中，在膀胱压力急剧上升的2秒内施加高频盆腔神经刺激（1-3 kHz， 1-2 mA正弦波形，持续60秒），表明即将出现排尿，能够抑制排尿。通过三种方式调节排尿：(1)抑制排尿（4只大鼠，n = 13试验）。没有流体输出或排出的流体体积非常小（<根据前2或3个空洞的平均值预期体积的15%）。排尿在整个刺激周期（60 s）内被抑制，并在停止刺激后37 s内恢复。(2)虚空延期（4只大鼠，n = 6试验）。即将出现的空洞被抑制（没有液体排出），但在刺激后期（刺激开始后12-44秒，平均24.5±5.2秒）出现了空洞。(3)减少空腔体积（5只大鼠，n = 20试验）。排空发生，但排空的液体体积为前两次或三次排空平均预期体积的15-80%（范围21.8-77.8%，平均45.3±3.6%）。停止刺激后5分钟内自然排尿恢复。在填充阶段在空隙之间的刺激没有效果。实验表明，在即将排尿开始时对骨盆神经进行条件高频刺激可以抑制排尿。这种效果是迅速可逆的，没有任何不良的行为副作用。
Female Wistar rats were instrumented to record bladder pressure and to stimulate the left pelvic nerve. Repeated voids were induced by continuous infusion of saline into the bladder (11.2 ml/h) via a T-piece in the line to the bladder catheter. In each animal tested (n = 6) high frequency pelvic nerve stimulation (1–3 kHz, 1–2 mA sinusoidal waveform for 60 s) applied within 2 s of the onset of a sharp rise in bladder pressure signaling an imminent void was able to inhibit micturition. Voiding was modulated in three ways: (1) Suppression of voiding (four rats, n = 13 trials). No fluid output or a very small volume of fluid expelled (<15% of the volume expected based on the mean of the previous 2 or 3 voids). Voiding suppressed for the entirety of the stimulation period (60 s) and resumed within 37 s of stopping stimulation. (2) Void deferred (four rats, n = 6 trials). The imminent void was suppressed (no fluid expelled) but a void occurred later in the stimulation period (12–44 s, mean 24.5 ± 5.2 s after the onset of the stimulation). (3) Reduction in voided volume (five rats, n = 20 trials). Voiding took place but the volume of fluid voided was 15–80% (range 21.8–77.8%, mean 45.3 ± 3.6%) of the volume expected from the mean of the preceding two or three voids. Spontaneous voiding resumed within 5 min of stopping stimulation. Stimulation during the filling phase in between voids had no effect. The experiments demonstrate that conditional high frequency stimulation of the pelvic nerve started at the onset of an imminent void can inhibit voiding. The effect was rapidly reversible and was not accompanied by any adverse behavioral side effects.
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影响因子：
2.7
作者：
Grissom, Nicola;Kerr, Wesley;Bhatnagar, Seema
通讯作者：
Bhatnagar, Seema
影响因子：
2.5
作者：
HULSEBOSCH, CE;COGGESHALL, RE
通讯作者：
COGGESHALL, RE
影响因子：
2
作者：
De Wachter, Stefan
通讯作者：
De Wachter, Stefan
影响因子：
2
作者：
Monjotin, Nicolas;Farrie, Martine;Junquero, Didier
通讯作者：
Junquero, Didier
DOI：
10.1254/jjp.52.405
发表时间：
1990-03-01
期刊：
JAPANESE JOURNAL OF PHARMACOLOGY
影响因子：
--
作者：
MORIKAWA, K;KAKIUCHI, M;GOMI, Y
通讯作者：
GOMI, Y