Paeoniflorin prevents postoperative peritoneal adhesion formation in an experimental rat model.
Paeoniflorin prevents postoperative peritoneal adhesion formation in an experimental rat model.
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DOI：
10.18632/oncotarget.21333
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发表时间：
2017-11-07
期刊：
影响因子：
--
通讯作者：
Li X
中科院分区：
文献类型：
--
作者：
Gao Q;Wei G;Wu Y;Yao N;Zhou C;Wang K;Wang K;Sun X;Li X
虽然材料和现代外科技术已经被开发出来以抑制术后粘连，但粘连仍有可能发生，因此，迫切需要一种新的有效的抗粘连药物。在本研究中，我们探讨了芍药苷对术后腹膜粘连的治疗效果，并检测了PE的抗氧化应激和抗炎特性。将48只雄性SD大鼠随机分为6组：假手术组、对照组、透明质酸组和3个浓度(10、20、40 mg/kg/d)的芍药苷组。腹部粘连是盲肠及其对侧腹壁磨损造成的。芍药苷组大鼠每日灌胃给药，连续7d。用U形切口重新切开大鼠的腹腔，对粘连进行大体分级。进行组织学分析，检测氧化应激、炎症细胞因子、胶原纤维降解和细胞角蛋白水平。肉眼和组织病理学测量显示，芍药苷减少了腹膜粘连和炎症。值得注意的是，芍药苷治疗降低了转化生长因子-β1、IL-6和COX-2的蛋白水平。PE组胶原纤维含量明显低于对照组。Western blotting分析显示，芍药苷可增加基质金属蛋白酶-9和超氧化物歧化酶-2的蛋白表达，显著降低α-SMA和COX-2的蛋白表达。芍药苷处理的动物腹膜间皮细胞更为连续和完整。我们的研究提示，在术后期间，芍药苷可以通过抗氧化应激和抗炎作用来改善腹膜粘连。
Although materials and modern surgical techniques have been developed to suppress postoperative adhesions, adhesion formation can still occur, and thus, a novel effective anti-adhesion drug is greatly needed. In the present study, we explored the efficacy of paeoniflorin treatment against postoperative peritoneal adhesions and examined the anti-oxidative stress and anti-inflammatory properties of PE. Forty-eight male Sprague-Dawley rats were randomly divided into 6 groups for the study: the sham, control, hyaluronan and three concentrations (10, 20 and 40 mg/kg/d) paeoniflorin groups. Abdominal adhesions were created by abrasion of the caecum and its opposite abdominal wall. In the paeoniflorin groups, the rats were administered daily oral doses of paeoniflorin for 7 days. The abdominal cavities of the rats were reopened with a U-shaped incision to macroscopically grade the adhesions. Histologic analysis was performed, and oxidative stress, inflammatory cytokine, collagen fiber degradation and cytokeratin levels were measured. Macroscopic and histopathological measurements revealed that paeoniflorin reduced peritoneal adhesion and inflammation. Notably, treatment with paeoniflorin reduced the protein levels of TGF-β1, IL-6 and COX-2. The collagen fiber fractions were distinctly lower in the PE groups than in the control group. Western blotting analyses showed that paeoniflorin increased MMP-9 and superoxide dismutase-2 protein expression and sharply reduced α-SMA and COX-2 protein expression. Peritoneal mesothelium cells were more continuous and complete in animals treated with paeoniflorin. Our study suggests that paeoniflorin can be used to ameliorate peritoneal adhesions via anti-oxidative stress and anti-inflammatory actions during the postoperative period.
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