Moxibustion therapy improving delayed memory deficits via promoting neurogenesis and angiogenesis of hippocampus in a vascular dementia rat model
Moxibustion therapy improving delayed memory deficits via promoting neurogenesis and angiogenesis of hippocampus in a vascular dementia rat model
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艾灸疗法通过促进血管性痴呆大鼠模型海马神经发生和血管生成改善迟发性记忆缺陷
DOI：
10.1007/s11726-019-1140-9
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发表时间：
2019-12
影响因子：
0.5
通讯作者：
Li Gui
中科院分区：
文献类型：
--
作者：
Yin-qiu Fan;Jun Yang;Jing-cheng Cui;Pin Wang;Yue Li;Li Gui
目的：探讨艾灸治疗后血管性痴呆大鼠延迟记忆的改变及其与神经发生和血管生成的关系。方法：选用成年雄性SPF级Wistar大鼠200只。随机选取36只大鼠为假手术组。除假手术组(n=36)外，其余大鼠采用双侧颈总动脉结扎(BCCAo)法制备VD模型。造模后将存活的108只大鼠随机分为3组：模型组、神经干细胞+内皮祖细胞艾灸组和神经干细胞艾灸组。神经干细胞+内皮祖细胞艾灸组大鼠植入联合移植的种植体，神经干细胞艾灸组仅移植神经干细胞。神经干细胞加内皮祖细胞艾灸组和神经干细胞艾灸组采用悬灸治疗百会、大椎、神庭穴(每组按疗程分为1、2、3个疗程)。各组于治疗3个疗程后用Morris水迷宫测定其延迟记忆能力，治疗1，2，3个疗程后分别灌胃4%多聚甲醛1d后取出脑组织。激光共聚焦显微镜检测标记蛋白表达，分析其对神经再生和血管生成的影响。结果：VD大鼠在缺血3d后Morris水迷宫实验中出现记忆延迟。艾灸治疗3个疗程后，神经干细胞加内皮祖细胞艾灸组和艾灸神经干细胞组的VD大鼠的迟发性记忆障碍均有改善。艾灸各疗程后，两组大鼠海马巢蛋白、双皮质素(DCX)和CD34的表达均显著增加(均P<0.05)，可能参与了海马区的神经发生和血管生成。此外，与神经干细胞艾灸组相比，艾灸神经干细胞+内皮祖细胞组大鼠脑内Nestin、DCX和CD34的表达显著增加(P<；0.05)。结论：艾灸可以逆转VD大鼠的迟发性记忆障碍，其机制可能与促进神经发生和血管生成有关。
Objective: To investigate the alteration of delayed memory and its relationship with neurogenesis and angiogenesis in vascular dementia (VD) rats after moxibustion therapy.Methods: Two hundred adult male SPF Wistar rats were chosen for the experiment. Thirty-six rats were randomly selected as the sham operation group. Except for rats in the sham operation group (n= 36), the others were made into VD models by bilateral common carotid arteries occlusion (BCCAo). After modeling, the 108 survived rats were randomly divided into 3 groups: a model group, a neural stem cells (NSCs) plus endothelial progenitor cells (EPCs) moxibustion group and a NSCs moxibustion group. Co-transplanted implant was transplanted into the rats in the NSCs plus EPCs moxibustion group, and the rats in the NSCs moxibustion group were transplanted by NSCs only. The NSCs plus EPCs moxibustion group and the NSCs moxibustion group received suspended moxibustion therapy at Baihui (GV 20), Dazhui (GV 14) and Shenting (GV 24),(each group was divided into 3 subgroups by the treatment course as 1, 2 and 3 courses). Every group was measured by Morris water maze to evaluate its delayed memory after 3 treatment courses and the rat’s brain was taken out after perfusion of 4% paraformaldehyde one day after 1, 2 and 3 treatment courses, respectively. Marker protein expression was detected by laser confocal microscope to analyze the effect on neurogenesis and angiogenesis.Results: VD rats showed delayed memory in Morris water maze test 3 d after ischemic injury. After 3 courses of moxibustion therapy, VD-induced delayed memory deficits were improved in the NSCs plus EPCs moxibustion group and the NSCs moxibustion group. The expressions of nestin, doublecortin (DCX) and CD34 increased significantly in the two moxibusiton groups after every treatment course (all P< 0.05), which might contribute to the neurogenesis and angiogenesis in hippocampus. In addition, compared with the rats in the NSCs moxibustion group, the expressions of nestin, DCX and CD34 increased significantly in the NSCs plus EPCs moxibustion group (P< 0.05).Conclusion: Moxibustion can reverse VD-induced delayed memory deficits, which may be related to the promotion of neurogenesis and angiogenesis.
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DOI：
--
发表时间：
2015-04
期刊：
Zhen ci yan jiu = Acupuncture research
影响因子：
--
作者：
Jian Zhao;Rui Huang;Huai-bin Li;K. Xiong
通讯作者：
Jian Zhao;Rui Huang;Huai-bin Li;K. Xiong
针对成人神经发生进行中风后治疗
影响因子：
4.3
作者：
Lu J;Manaenko A;Hu Q
通讯作者：
Hu Q
影响因子：
6.1
作者：
Yoo DY;Lee KY;Park JH;Jung HY;Kim JW;Yoon YS;Won MH;Choi JH;Hwang IK
通讯作者：
Hwang IK
影响因子：
5.3
作者：
Ohab, John J.;Fleming, Sheila;Carmichael, S. Thomas
通讯作者：
Carmichael, S. Thomas
影响因子：
4.4
作者：
Okazaki, T;Ebihara, S;Yamaya, M
通讯作者：
Yamaya, M