Conjugation to nickel-chelating nanolipoprotein particles increases the potency and efficacy of subunit vaccines to prevent West Nile encephalitis.
Conjugation to nickel-chelating nanolipoprotein particles increases the potency and efficacy of subunit vaccines to prevent West Nile encephalitis.
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DOI：
10.1021/bc100083d
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发表时间：
2010-06-16
影响因子：
4.7
通讯作者：
Mason PW
中科院分区：
文献类型：
--
作者：
Fischer NO;Infante E;Ishikawa T;Blanchette CD;Bourne N;Hoeprich PD;Mason PW
亚单位抗原是疫苗开发的有吸引力的候选者，因为它们是安全的、成本有效的和快速生产的。然而，亚单位抗原通常需要佐剂化和/或配制以产生具有可接受的效力和功效的产品。在这里，我们描述了一种简单的方法，用于提高重组亚单位抗原的效价和功效，通过将其固定在镍螯合纳米脂蛋白颗粒（NiNLP）上。NiNLP是膜模拟纳米颗粒，其提供适合于结合以聚组氨酸标签为特征的任何重组亚基免疫原的递送和呈递平台。将His标记的可溶性截短形式的西尼罗病毒（WNV）包膜蛋白（trE-His）固定在NiNLP上。NiNLP-trE-His的单次接种产生了上级抗WNV免疫应答，并且与单独接种trE-His的小鼠相比，提供了针对活WNV攻击的显著改善的保护。这些结果在疫苗开发和优化中具有广泛的意义，因为NiNLP技术非常适合于许多类型的疫苗，为增强重组亚单位免疫原的效力和功效提供了通用平台。
Subunit antigens are attractive candidates for vaccine development as they are safe, cost-effective, and rapidly produced. Nevertheless, subunit antigens often need to be adjuvanted and/or formulated to produce products with acceptable potency and efficacy. Here we describe a simple method for improving the potency and efficacy of a recombinant subunit antigen by its immobilization on nickel-chelating nanolipoprotein particles (NiNLPs). NiNLPs are membrane mimetic nanoparticles that provide a delivery and presentation platform amenable to binding any recombinant subunit immunogens featuring a poly-histidine tag. A His-tagged, soluble truncated form of the West Nile virus (WNV) envelope protein (trE-His) was immobilized on NiNLPs. Single inoculations of the NiNLP-trE-His produced superior anti-WNV immune responses and provided significantly improved protection against a live WNV challenge compared to mice inoculated with trE-His alone. These results have broad implications in vaccine development and optimization, as NiNLP technology is well suited to many types of vaccines, providing a universal platform for enhancing the potency and efficacy of recombinant subunit immunogens.
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影响因子：
3.7
作者：
KONISHI, E;PINCUS, S;MASON, PW
通讯作者：
MASON, PW
影响因子：
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作者：
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作者：
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影响因子：
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作者：
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通讯作者：
Hoeprich, Paul D.
影响因子：
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作者：
Katzen, Federico;Fletcher, Julia E.;Kudlicki, Wieslaw
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