HEADLESS, a WUSCHEL homolog, uncovers novel aspects of shoot meristem regulation and leaf blade development in Medicago truncatula.
HEADLESS, a WUSCHEL homolog, uncovers novel aspects of shoot meristem regulation and leaf blade development in Medicago truncatula.
复制标题
HEADLESS 是 WUSCHEL 同源物，揭示了蒺藜苜蓿芽分生组织调节和叶片发育的新方面
DOI：
10.1093/jxb/ery346
复制
发表时间：
2019-01-01
影响因子：
6.9
通讯作者：
Lin H
中科院分区：
文献类型：
--
作者：
Meng Y;Liu H;Wang H;Liu Y;Zhu B;Wang Z;Hou Y;Zhang P;Wen J;Yang H;Mysore KS;Chen J;Tadege M;Niu L;Lin H
WOX家族转录因子HEADLESS在蒺藜苜蓿的茎分生组织和叶片形态调控中具有抑制活性和保守的新功能。茎顶端分生组织（SAM）的形成和维持对植物的发育至关重要。然而，在模式豆科植物蒺藜苜蓿中，调节分生组织细胞活性的潜在分子机制知之甚少。利用正向遗传学方法，我们鉴定了拟南芥WUSCHEL的同源物HEADLESS（HDL），它是拟南芥SAM维持和叶片发育所必需的。蒺藜HDL的破坏导致SAM和腋生分生组织的无序规范和逮捕，导致HDL突变体被锁定在营养期没有明显的茎伸长。hdl突变体的叶在近端-远端轴上较短，这是由于叶长伸长减少，这导致更高的叶片宽/长比和改变的叶形状，揭示了拟南芥wus突变体中未描述的新表型。HDL通过其保守的WUS盒和EAR样基序募集MtTPL作为转录抑制因子发挥作用。进一步的遗传分析表明，HDL和STF是M.尽管HDL可以以与STF相同的方式募集MtTPL，但在叶片发育中独立起作用。我们的研究结果表明，HDL在调控紫茎尖分生组织和叶片形态方面具有保守和新颖的功能。truncatula，为了解分生组织生物学和植物发育提供了新的途径。
The WOX family transcription factor HEADLESS exhibits a repressive activity and performs conserved and novel functions in the regulation of shoot meristems and leaf shape in Medicago truncatula. The formation and maintenance of the shoot apical meristem (SAM) are critical for plant development. However, the underlying molecular mechanism of regulating meristematic cell activity is poorly understood in the model legume Medicago truncatula. Using forward genetic approaches, we identified HEADLESS (HDL), a homolog of Arabidopsis WUSCHEL, required for SAM maintenance and leaf development in M. truncatula. Disruption of HDL led to disorganized specification and arrest of the SAM and axillary meristems, resulting in the hdl mutant being locked in the vegetative phase without apparent stem elongation. hdl mutant leaves are shorter in the proximal–distal axis due to reduced leaf length elongation, which resulted in a higher blade width/length ratio and altered leaf shape, uncovering novel phenotypes undescribed in the Arabidopsis wus mutant. HDL functions as a transcriptional repressor by recruiting MtTPL through its conserved WUS-box and EAR-like motif. Further genetic analysis revealed that HDL and STENOFOLIA (STF), a key regulator of M. truncatula lamina outgrowth, act independently in leaf development although HDL could recruit MtTPL in the same manner as STF does. Our results indicate that HDL has conserved and novel functions in regulating shoot meristems and leaf shape in M. truncatula, providing new avenues for understanding meristem biology and plant development.
登录
查看更多内容
影响因子：
56.9
作者：
Fletcher, LC;Brand, U;Meyerowitz, EM
通讯作者：
Meyerowitz, EM
影响因子：
27.5
作者：
Dolzblasz, Alicja;Nardmann, Judith;Laux, Thomas
通讯作者：
Laux, Thomas
影响因子：
56.9
作者：
Kondo, Tatsuhiko;Sawa, Shinichiro;Sakagami, Youji
通讯作者：
Sakagami, Youji
影响因子：
11.8
作者：
Busch, Wolfgang;Miotk, Andrej;Lohmann, Jan U.
通讯作者：
Lohmann, Jan U.
影响因子：
11.6
作者：
Ikeda, Miho;Mitsuda, Nobutaka;Ohme-Takagi, Masaru
通讯作者：
Ohme-Takagi, Masaru