Trabecular bone histomorphometry in humans with Type 1 Diabetes Mellitus.
Trabecular bone histomorphometry in humans with Type 1 Diabetes Mellitus.
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DOI：
10.1016/j.bone.2011.09.055
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发表时间：
2012-01
期刊：
影响因子：
4.1
通讯作者：
Recker, Robert R.
中科院分区：
文献类型：
--
作者：
Armas, Laura A. G.;Akhter, Mohammed P.;Drincic, Andjela;Recker, Robert R.
1型糖尿病(DM)患者发生骨折的风险显著增加，但对于骨骼微结构或重塑特性的异常可能有助于了解这些患者骨骼脆性的发病机制，人们知之甚少。我们报告了一项病例对照研究，比较了18名患有1型糖尿病的健康受试者和年龄和性别匹配的健康非糖尿病受试者的髂骨活检的骨组织形态计量学和显微CT结果。其中五名糖尿病患者有低创伤骨折的病史。四环素标记后取骨活检标本。活检标本用16微米分辨率的台式μCT进行固定、包埋和扫描。然后对它们进行切片，并按照Recker等人之前的描述进行定量组织形态计量学。1988年。从每个活检的中心部分读取两个相距250μm的切片。总体而言，糖尿病患者和对照组在组织形态计量学或显微CT测量方面没有显着差异。然而，在这两种分析方法中，骨折糖尿病患者的结构和动态趋势与非骨折糖尿病患者不同。总而言之，在没有明显并发症的情况下，1型糖尿病不会导致骨组织形态计量学或显微CT变量的异常。然而，患有骨折的糖尿病患者的骨骼微结构可能存在缺陷，这可能是骨骼过度脆弱的原因。
Patients with Type 1 Diabetes Mellitus (DM) have markedly increased risk of fracture, but little is known about abnormalities in bone micro-architecture or remodeling properties that might give insight into the pathogenesis of skeletal fragility in these patients. We report here a case-control study comparing bone histomorphometric and micro-CT results from iliac biopsies in 18 otherwise healthy subjects with Type 1 Diabetes Mellitus with those from healthy age- and sex- matched non-diabetic control subjects. Five of the diabetics had histories of low-trauma fracture. Transilial bone biopsies were obtained after tetracycline labeling. The biopsy specimens were fixed, embedded, and scanned using a desktop μCT at 16 micron resolution. They were then sectioned and quantitative histomorphometry was performed as previously described by Recker et al. 1988. Two sections, >250 μm apart, were read from the central part of each biopsy. Overall there were no significant differences between diabetics and controls in histomorphometric or micro-CT measurements. However, fracturing diabetics had structural and dynamic trends different from nonfracturing diabetics by both methods of analysis. In conclusion, Type 1 Diabetes Mellitus does not result in abnormalities in bone histomorphometric or micro-CT variables in the absence of manifest complications from the diabetes. However, diabetics suffering fractures may have defects in their skeletal microarchitecture that may underlie the presence of excess skeletal fragility.
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DOI：
10.1359/jbmr.080713
发表时间：
2008-12
期刊：
Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research
影响因子：
--
作者：
Moyer-Mileur LJ;Slater H;Jordan KC;Murray MA
通讯作者：
Murray MA
影响因子：
16.2
作者：
American Diabetes Association
通讯作者：
American Diabetes Association
影响因子：
15.9
作者：
ISLEY, WL;UNDERWOOD, LE;CLEMMONS, DR
通讯作者：
CLEMMONS, DR
影响因子：
8.2
作者：
Forsén, L;Meyer, HE;Edna, TH
通讯作者：
Edna, TH
影响因子：
3.8
作者：
Bridges, MJ;Moochhala, SH;Kelly, CA
通讯作者：
Kelly, CA