Directing tissue morphogenesis via self-assembly of vascular mesenchymal cells.
Directing tissue morphogenesis via self-assembly of vascular mesenchymal cells.
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DOI：
10.1016/j.biomaterials.2012.08.067
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发表时间：
2012-12
期刊：
影响因子：
14
通讯作者：
Ho CM
中科院分区：
文献类型：
--
作者：
Chen TH;Zhu X;Pan L;Zeng X;Garfinkel A;Tintut Y;Demer LL;Zhao X;Ho CM
为再生医学重建受损组织需要复制模仿自然形态的组织/生物材料的技术。为了重建组织模式，目前的方法包括使用具有特定结构的支架来接种细胞，引导细胞扩散或直接将细胞移动到所需位置。然而，结构复杂性是有限的。此外，人工定义的模式通常被随后的组织发育中的细胞自组织（例如细胞迁移和细胞-细胞通信）打乱。在这里，通过与细胞自组织合作，而不是反对它，我们实验和数学证明了一种方法，指导自组织血管间充质细胞（VMC）组装成所需的多细胞模式。通过与微工程基底和VMC的自发聚集的界面作用揭示了VMC的固有手性，初始细胞铺板的分布差异可以被放大成分别模仿肝小叶或骨单位的横截面结构的精致的放射状结构或同心环的形成。此外，当与VMC共培养时，非图案形成内皮细胞沿着VMC沿着并以协调的方式形成连贯的放射状或环状图案，表明对异型细胞组织的适用性。
Rebuilding injured tissue for regenerative medicine requires technologies to reproduce tissue/biomaterials mimicking the natural morphology. To reconstitute the tissue pattern, current approaches include using scaffold with specific structure to plate cells, guiding cell spreading, or directly moving cells to desired locations. However, the structural complexity is limited. Also, the artificially-defined patterns are usually disorganized by cellular self-organization in the subsequent tissue development, such as cell migration and cell-cell communication. Here, by working in concert with cellular self-organization rather than against it, we experimentally and mathematically demonstrate a method which directs self-organizing vascular mesenchymal cells (VMCs) to assemble into desired multicellular patterns. Incorporating the inherent chirality of VMCs revealed by interfacing with micro-engineered substrates and VMCs’ spontaneous aggregation, difference in distribution of initial cell plating can be amplified into the formation of exquisite radial structures or concentric rings mimicking the cross-sectional structure of liver lobules or osteons, respectively. Furthermore, when co-cultured with VMCs, non-pattern-forming endothelial cells tracked along the VMCs and formed a coherent radial or ring pattern in a coordinated manner, indicating the applicability to heterotypical cell organization.
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影响因子：
3.7
作者：
DIMILLA, PA;QUINN, JA;LAUFFENBURGER, DA
通讯作者：
LAUFFENBURGER, DA
影响因子：
--
作者：
Maini, PK;McElwain, DLS;Leavesley, DI
通讯作者：
Leavesley, DI
DOI：
10.1073/pnas.0308436101
发表时间：
2004-06-22
影响因子：
11.1
作者：
Garfinkel, A;Tintut, Y;Demer, LL
通讯作者：
Demer, LL
DOI：
10.1098/rstb.1952.0012
发表时间：
1952-01-01
期刊：
PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY OF LONDON SERIES B-BIOLOGICAL SCIENCES
影响因子：
--
作者：
TURING, AM
通讯作者：
TURING, AM
影响因子：
6.1
作者：
Li N;Ho CM
通讯作者：
Ho CM