Microbial Similarity and Preference for Specific Sites in Healthy Oral Cavity and Esophagus.
Microbial Similarity and Preference for Specific Sites in Healthy Oral Cavity and Esophagus.
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健康口腔和食道特定部位的微生物相似性和偏好
DOI：
10.3389/fmicb.2018.01603
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发表时间：
2018
影响因子：
5.2
通讯作者：
Wei WQ
中科院分区：
文献类型：
--
作者：
Dong L;Yin J;Zhao J;Ma SR;Wang HR;Wang M;Chen W;Wei WQ
人类微生物群落是高度复杂的生态系统，但目前尚不清楚口腔和食道不同部位的微生物组成是否有任何相似性。临床标本取自口腔三个壁龛(唾液、舌背和牙龈上菌斑)和食道三段(上、中、下)，共27例。在Illumina测序平台上扩增细菌16S rRNA基因V3-V4区序列，并用QIIME和LEFSE软件进行数据分析。口腔有29门365属的细菌，食道有29门594属的细菌。变形杆菌、拟杆菌、放线杆菌、梭杆菌和TM7在口腔和食道中含量最高，但放线杆菌和拟杆菌在口腔和食道中的含量最高。口腔和食道均以链球菌属、奈瑟氏菌属、普氏杆菌属、放线杆菌属和韦龙氏菌属最多，口腔以奈瑟氏菌属为主，食道以链球菌属为主。在口腔中发现了不同的生态位特异性细菌特征，例如在牙周上菌斑中发现了黄杆菌类，在唾液中发现了类杆菌，在舌背中发现了梭状芽胞杆菌。相比之下，没有发现三个不同食道段的部位特异性细菌。然而，观察到个体之间微生物组成的高度变异性。综上所述，本研究证实了健康口腔和食道中不同分类水平的微生物多样性，并在上消化道的六个壁龛中确定了对位置有利的细菌特征。这些发现为未来解释微生物组相关疾病的研究提供了关键的基线。
Human microbial communities are highly complex ecosystems, but it remains unclear if microbial compositions have any similarity in distinct sites of the oral cavity and esophagus in particular. Clinical samples were collected from three niches (saliva, tongue dorsum and supragingival plaque) of the oral cavity and three segments (upper, middle, and lower) of the esophagus in 27 healthy individuals. Bacterial V3-V4 region of 16S rRNA gene in these samples was amplified and sequenced on Illumina sequencing platform, followed by data analysis using QIIME and LEfSe softwares. Highly diverse bacterial flora with 365 genera belonging to 29 phyla resided in the oral cavity and 594 genera belonging to 29 phyla in the esophagus. The phyla Proteobacteria, Firmicutes, Bacteroidetes, Actinobacteria, Fusobacteria, and TM7 were most abundant in both the oral cavity and the esophagus, but the phyla Actinobacteria and Bacteroidetes were preferable in the oral cavity and Firmicutes in the esophagus. The genera Streptococcus, Neisseria, Prevotella, Actinobacillus, and Veillonella were most abundant in both oral cavity and esophagus, but Neisseria was preferable in the oral cavity and Streptococcus in the esophagus. Different niche-specific bacterial signatures were found in the oral cavity, e.g., the class Flavobacteria in the supragingival plaque, class Bacteroides in the saliva and the class Clostridia in the tongue dorsum. By contrast, no site specific bacteria for three different segments of esophagus were found. However, high variability of microbial compositions between individuals was observed. In conclusion, this study confirmed microbial diversity at different taxonomic levels in healthy oral cavity and esophagus, and identified the site-preferable bacterial signatures in six niches of the upper digestive tract. These findings provide a critical baseline for future studies interpreting microbiome-related diseases.
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影响因子：
4.9
作者：
Kelsen J;Bittinger K;Pauly-Hubbard H;Posivak L;Grunberg S;Baldassano R;Lewis JD;Wu GD;Bushman FD
通讯作者：
Bushman FD
影响因子：
7
作者：
Nasidze, Ivan;Li, Jing;Stoneking, Mark
通讯作者：
Stoneking, Mark
影响因子：
3.1
作者：
GIBBONS, RJ;SPINELL, DM;SKOBE, Z
通讯作者：
SKOBE, Z
DOI：
10.1038/ismej.2010.30
发表时间：
2010-08
期刊：
The ISME journal
影响因子：
--
作者：
通讯作者：
--
DOI：
10.1073/pnas.1000080107
发表时间：
2011-03-15
影响因子：
11.1
作者：
Caporaso, J. Gregory;Lauber, Christian L.;Knight, Rob
通讯作者：
Knight, Rob