Overexpression of luxS Promotes Stress Resistance and Biofilm Formation of Lactobacillus paraplantarum L-ZS9 by Regulating the Expression of Multiple Genes.

Overexpression of luxS Promotes Stress Resistance and Biofilm Formation of Lactobacillus paraplantarum L-ZS9 by Regulating the Expression of Multiple Genes.
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luxS过表达通过调节多基因表达促进副植物乳杆菌L-ZS9的应激抵抗和生物膜形成

DOI:
10.3389/fmicb.2018.02628
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发表时间:
2018
影响因子:
5.2
通讯作者:
Li P
Li P
中科院分区:
生物学2区
文献类型:
--
作者:
Liu L;Wu R;Zhang J;Li P

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近年来,益生菌因其有益的作用引起了人们的极大兴趣。本研究旨在探讨luxS过表达是否促进了拟植物乳杆菌L-ZS9的抗逆性。本研究表明,luxS基因的过表达增加了拟植物L-ZS9的自诱导剂2 (AI-2,群体感应信号分子)的产生。同时,luxS的过表达促进了菌株的耐热性、耐胆盐性和生物膜的形成。RNAseq结果表明,luxS可调控转运蛋白、膜蛋白和转录调节因子的多个基因。这些结果揭示了LuxS在促进益生菌发酵剂抗逆性和生物膜形成方面的新作用。
Probiotics have evoked great interest in the past years for their beneficial effects. The aim of this study was to investigate whether luxS overexpression promotes the stress resistance of Lactobacillus paraplantarum L-ZS9. Here we show that overexpression of luxS gene increased the production of autoinducer-2 (AI-2, quorum sensing signal molecule) by L. paraplantarum L-ZS9. At the same time, overexpression of luxS promoted heat-, bile salt-resistance and biofilm formation of the strain. RNAseq results indicated that multiple genes encoding transporters, membrane proteins, and transcriptional regulator were regulated by luxS. These results reveal a new role for LuxS in promoting stress resistance and biofilm formation of probiotic starter.
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