Biosensor capability of the endometrium is mediated in part, by altered miRNA cargo from conceptus-derived extracellular vesicles
Biosensor capability of the endometrium is mediated in part, by altered miRNA cargo from conceptus-derived extracellular vesicles
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子宫内膜的生物传感器能力部分是由来自孕体来源的细胞外囊泡的改变的 miRNA 货物介导的
DOI：
10.1101/2023.10.27.564369
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发表时间：
2023
期刊：
影响因子：
--
通讯作者：
De Bem T
中科院分区：
文献类型：
--
作者：
De Bem T
我们验证了子宫内膜的生物传感器能力部分是由孕妇在妊娠着床期分泌的细胞外囊泡中所含的不同货物所介导的假设。我们将不同来源的牛牛胚胎，体内（高发育潜力（IV）），体外（中等发育潜力（IVF））或克隆（低发育潜力（NT））移植到牛牛indicus受体中。从第16天的受孕条件培养基中恢复的细胞外囊泡（ev）进行了表征，并对其microRNA （miRNA）货物与各自子宫内膜的RNA测序进行了测序。子宫内膜对体内与体外、体内与克隆胚胎的反应存在显著差异（分别为1153和334g），体外与克隆胚胎的差异有限（36个deg）。在所有三组中，概念衍生ev中包含的miRNA货物相似（共有426个miRNA）。在体内和克隆胚胎之间只有8个mirna存在差异，而在体内和体外衍生的胚胎之间只有6个mirna存在差异。用miR‐128和miR‐1298的模拟物或抑制剂处理子宫内膜上皮细胞，改变了体内子宫内膜中mrna改变的靶细胞（分别为96和85）的蛋白质组学含量（PLXDC2, COPG1, HSPA12A, MCM5， TBL1XR1和ttf）。总之，我们已经确定子宫内膜的生物传感器能力在一定程度上是由其对妊娠着床期孕妇产生的不同ev miRNA货物的反应介导的。
We tested the hypothesis that the biosensor capability of the endometrium is mediated in part, by the effect of different cargo contained in the extracellular vesicles secreted by the conceptus during the peri‐implantation period of pregnancy. We transferredBos taurus taurusembryos of different origin, in vivo (high developmental potential (IV)), in vitro (intermediate developmental potential (IVF)), or cloned (low developmental potential (NT)), intoBos taurus indicusrecipients. Extracellular vesicles (EVs) recovered from Day 16 conceptus‐conditioned medium were characterized and their microRNA (miRNA) cargo sequenced alongside RNA sequencing of their respective endometria. There were substantial differences in the endometrial response to in vivo versus in vitro and in vivo versus cloned conceptuses (1153 and 334DEGs respectively) with limited differences between in vitro Vs cloned conceptuses (36 DEGs). The miRNA cargo contained in conceptus‐derived EVs was similar between all three groups (426 miRNA in common). Only 8 miRNAs were different between in vivo and cloned conceptuses, while only 6 miRNAs were different between in vivo and in vitro‐derived conceptuses. Treatment of endometrial epithelial cells with mimic or inhibitors for miR‐128 and miR‐1298 changed the proteomic content of target cells (96 and 85, respectively) of which mRNAs are altered in the endometrium in vivo (PLXDC2, COPG1, HSPA12A, MCM5, TBL1XR1, andTTF).In conclusion, we have determined that the biosensor capability of the endometrium is mediated in part, by its response to different EVs miRNA cargo produced by the conceptus during the peri‐implantation period of pregnancy.
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DOI：
10.1073/pnas.0811841106
发表时间：
2009-04-07
影响因子：
11.1
作者：
Bauersachs, Stefan;Ulbrich, Susanne E.;Wolf, Eckhard
通讯作者：
Wolf, Eckhard
牛发情周期期间子宫内膜转录组的变化：低循环黄体酮的影响及其对概念延长的影响1
影响因子：
3.6
作者：
N. Forde;M. Beltman;G. Duffy;P. Duffy;J. Mehta;P. O'Gaora;J. Roche;P. Lonergan;M. Crowe
通讯作者：
M. Crowe
影响因子：
3.6
作者：
Forde, Niamh;Mehta, Jai P.;Lonergan, Pat
通讯作者：
Lonergan, Pat
母体有机体和胚胎生物传感：反刍动物的见解。
影响因子：
3.4
作者：
O. Sandra;F. Constant;A. Vitorino Carvalho;C. Eozenou;D. Valour;V. Mauffré;I. Hue;G. Charpigny
通讯作者：
G. Charpigny
母牛妊娠识别过程中孕体与子宫内膜的串扰1
影响因子：
3.6
作者：
S. Mamo;J. Mehta;N. Forde;P. McGettigan;P. Lonergan
通讯作者：
P. Lonergan