Neuromesodermal progenitors are a conserved source of spinal cord with divergent growth dynamics.
Neuromesodermal progenitors are a conserved source of spinal cord with divergent growth dynamics.
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DOI：
10.1242/dev.166728
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发表时间：
2018-11-09
期刊：
影响因子：
--
通讯作者：
Steventon B
中科院分区：
文献类型：
--
作者：
Attardi A;Fulton T;Florescu M;Shah G;Muresan L;Lenz MO;Lancaster C;Huisken J;van Oudenaarden A;Steventon B
在原肠胚形成过程中，胚胎细胞分化为不同的胚层。在小鼠中，这在从称为神经中胚层祖细胞（NMps）的双能干细胞群体的体节发生中持续。然而，在快速发育的斑马鱼胚胎中，与NMps相关的自我更新程度尚不清楚。使用遗传克隆追踪方法，我们标记早期胚胎祖细胞，发现脊髓和中胚层组织之间有很强的克隆相似性。我们使用光片成像跟踪单个细胞谱系，揭示了跨前后体轴的脊髓组织子集的共同神经中胚层谱系贡献。最初的人口细分在中期原肠胚阶段，并直接分配到神经和中胚层隔室原肠胚形成。第二个人口在尾芽进行延迟分配，以促进神经和中胚层隔室只有在后期体节发生。在体节发生后期的细胞追踪和回顾性细胞命运分配揭示了这些细胞是单命运祖细胞的集合。我们的研究结果表明，NMPs是一个保守的人口的双能祖细胞，其谱系的变化在一个物种特异性的方式，由于分化和生长的速度有很大的不同。 斑马鱼神经中胚层祖细胞的完整谱系分析，通过遗传克隆标记，正向命运定位和全成像的组合。
During gastrulation, embryonic cells become specified into distinct germ layers. In mouse, this continues throughout somitogenesis from a population of bipotent stem cells called neuromesodermal progenitors (NMps). However, the degree of self-renewal associated with NMps in the fast-developing zebrafish embryo is unclear. Using a genetic clone-tracing method, we labelled early embryonic progenitors and found a strong clonal similarity between spinal cord and mesoderm tissues. We followed individual cell lineages using light-sheet imaging, revealing a common neuromesodermal lineage contribution to a subset of spinal cord tissue across the anterior-posterior body axis. An initial population subdivides at mid-gastrula stages and is directly allocated to neural and mesodermal compartments during gastrulation. A second population in the tailbud undergoes delayed allocation to contribute to the neural and mesodermal compartment only at late somitogenesis. Cell tracking and retrospective cell fate assignment at late somitogenesis stages reveal these cells to be a collection of mono-fated progenitors. Our results suggest that NMps are a conserved population of bipotential progenitors, the lineage of which varies in a species-specific manner due to vastly different rates of differentiation and growth. A complete lineage analysis of neuromesodermal progenitors in zebrafish through a combination of genetic clonal labelling, forward fate mapping and in toto imaging.
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影响因子：
9.2
作者：
Brown, JM;Storey, KG
通讯作者：
Storey, KG
DOI：
10.1016/j.cub.2009.01.052
发表时间：
2009-03-10
期刊：
Current biology : CB
影响因子：
--
作者：
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通讯作者：
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影响因子：
11.8
作者：
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通讯作者：
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影响因子：
64.8
作者：
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通讯作者：
van Oudenaarden, Alexander
影响因子：
7.7
作者：
Wymeersch FJ;Huang Y;Blin G;Cambray N;Wilkie R;Wong FC;Wilson V
通讯作者：
Wilson V