The C. elegans dosage compensation complex propagates dynamically and independently of X chromosome sequence.
The C. elegans dosage compensation complex propagates dynamically and independently of X chromosome sequence.
复制标题
DOI：
10.1016/j.cub.2009.09.047
复制
发表时间：
2009-11-17
期刊：
影响因子：
--
通讯作者：
Lieb JD
中科院分区：
文献类型：
--
作者：
Ercan S;Dick LL;Lieb JD
秀丽隐杆线虫剂量补偿复合物（DCC）与XX型动物的两条X染色体结合，以降低X连锁转录本的水平。五个DCC成员与进化上保守的凝聚素复合物的亚基同源，而两个非凝聚素亚基是DCC募集到X染色体所必需的。在此，我们通过检测不同发育阶段以及含有X染色体 - 常染色体融合的品系中基因表达和DCC亚基的结合模式，研究了DCC募集以及沿X染色体扩散的分子机制。我们表明，DCC结合是根据发育过程中的基因活性动态确定的，并且DCC扩散的机制与X染色体DNA序列无关。因此，在X染色体 - 常染色体融合品系中，DCC扩散从X连锁募集位点传播到常染色体启动子上，这是距离的函数。对扩散的定量分析表明，凝聚素样亚基比参与初始X染色体靶向的亚基更容易从募集位点扩散到启动子。通过这些机制，一个高度保守的染色质复合物被用于在发育过程中实现区域规模的转录调控。与果蝇DCC所使用的X染色体识别和扩散策略的相似性表明了染色体规模基因调控的基本机制。
The C. elegans Dosage Compensation Complex (DCC) associates with both X chromosomes of XX animals to reduce X-linked transcript levels. Five DCC members are homologous to subunits of the evolutionarily conserved condensin complex, while two non-condensin subunits are required for DCC recruitment to X. Here, we investigated the molecular mechanism of DCC recruitment and spreading along X by examining gene expression and the binding patterns of DCC subunits in different stages of development, and in strains harboring X;autosome fusions. We show that DCC binding is dynamically specified according to gene activity during development, and that the mechanism of DCC spreading is independent of X-chromosome DNA sequence. Accordingly, in X;A fusion strains DCC spreading propagates from X-linked recruitment sites onto autosomal promoters as a function of distance. Quantitative analysis of spreading suggests that the condensin-like subunits spread from recruitment sites to promoters more readily than subunits involved in initial X-targeting. Via these mechanisms, a highly conserved chromatin complex is appropriated to accomplish domain-scale transcriptional regulation during development. Similarities to the X-recognition and spreading strategies used by the Drosophila DCC suggest mechanisms fundamental to chromosome-scale gene regulation.
登录
查看更多内容
影响因子：
64.5
作者：
Alekseyenko AA;Peng S;Larschan E;Gorchakov AA;Lee OK;Kharchenko P;McGrath SD;Wang CI;Mardis ER;Park PJ;Kuroda MI
通讯作者：
Kuroda MI
影响因子：
64.5
作者：
CHUANG, PT;ALBERTSON, DG;MEYER, BJ
通讯作者：
MEYER, BJ
影响因子：
56.9
作者：
Chuang, PT;Lieb, JD;Meyer, BJ
通讯作者：
Meyer, BJ
影响因子：
30.8
作者：
Ercan, Sevinc;Giresi, Paul G.;Lieb, Jason D.
通讯作者：
Lieb, Jason D.
影响因子：
14.9
作者：
Ji X;Li W;Song J;Wei L;Liu XS
通讯作者：
Liu XS