Does pre-enrichment of anodes with acetate to select for Geobacter spp. enhance performance of microbial fuel cells when switched to more complex substrates?
Does pre-enrichment of anodes with acetate to select for Geobacter spp. enhance performance of microbial fuel cells when switched to more complex substrates?
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DOI：
10.3389/fmicb.2023.1199286
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发表时间：
2023
影响因子：
5.2
通讯作者：
中科院分区：
文献类型：
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影响微生物燃料电池（MFC）性能的因素很多。电池配置和材料对MFC性能的影响已经引起了相当大的关注。关于阳极微生物群的影响的工作要少得多，特别是在使用复杂基质作为燃料的情况下。改善MFC在复杂基质如废水上的性能的一种策略是用已知的有效产电菌如地芽孢杆菌属（Geophysicspp.）这种策略的含义是，电原是MFC中的限制因素，而通过水解和发酵来供给电原的生物体是不受限制的。我们进行了系统的测试，这种策略和相关的假设it. Microbial燃料电池富集使用三种不同的基板（醋酸盐，合成废水和真实的生活污水）和三种不同的接种物（活性污泥，泰恩河沉积物，从MFC的流出物）。反应器从一开始就富集复杂的底物，或者最初进料乙酸盐以富集地芥菜属。在转换到合成或真实的废水之前。乙酸盐的预富集增加了土壤中Geophysp的相对丰度。与从实验开始就进料复合基质的MFC相比，在转换为复合基质的MFC中（废水进料的MFC- 21.9 ± 1.7%的Geolumspp.; - 富含乙酸盐的MFC，进料废水- 34.9 ± 6.7%的地芥菜属;合成废水进料的MFC- 42.5 ± 3.7%的Geatrium spp.;富含乙酸盐的合成废水进料的MFC- 47.3 ± 3.9%的Geatron spp.）。然而，乙酸盐预富集没有转化为电池电压、最大电流密度、最大功率密度或底物去除效率的显著改善。然而，当在乙酸盐富集后补加复杂底物时，在乙酸盐预富集的MFC中的库仑效率（CE）较高（废水进料的MFC- CE = 22.0 ± 6.2%;乙酸盐富集的MFC，进料废水- CE =58.5 ± 3.5%;合成废水进料的MFC- CE = 22.0 ± 3.2%;富含乙酸盐的合成废水进料MFC- 28.7 ± 4.2%。的相对丰度Gealesssp。CE代表每种基质用三种不同接种物接种的九个重复反应器的平均值。因此，利用复杂有机基质提高MFC中阳极微生物群落性能的努力应集中在提高驱动水解和发酵的生物体的活性，而不是末端氧化产电菌。
Many factors affect the performance of microbial fuel cells (MFCs). Considerable attention has been given to the impact of cell configuration and materials on MFC performance. Much less work has been done on the impact of the anode microbiota, particularly in the context of using complex substrates as fuel. One strategy to improve MFC performance on complex substrates such as wastewater, is to pre-enrich the anode with known, efficient electrogens, such as Geobacter spp. The implication of this strategy is that the electrogens are the limiting factor in MFCs fed complex substrates and the organisms feeding the electrogens through hydrolysis and fermentation are not limiting. We conducted a systematic test of this strategy and the assumptions associated with it. Microbial fuel cells were enriched using three different substrates (acetate, synthetic wastewater and real domestic wastewater) and three different inocula (Activated Sludge, Tyne River sediment, effluent from an MFC). Reactors were either enriched on complex substrates from the start or were initially fed acetate to enrich for Geobacter spp. before switching to synthetic or real wastewater. Pre-enrichment on acetate increased the relative abundance of Geobacter spp. in MFCs that were switched to complex substrates compared to MFCs that had been fed the complex substrates from the beginning of the experiment (wastewater-fed MFCs - 21.9 ± 1.7% Geobacter spp.; acetate-enriched MFCs, fed wastewater - 34.9 ± 6.7% Geobacter spp.; Synthetic wastewater fed MFCs – 42.5 ± 3.7% Geobacter spp.; acetate-enriched synthetic wastewater-fed MFCs - 47.3 ± 3.9% Geobacter spp.). However, acetate pre-enrichment did not translate into significant improvements in cell voltage, maximum current density, maximum power density or substrate removal efficiency. Nevertheless, coulombic efficiency (CE) was higher in MFCs pre-enriched on acetate when complex substrates were fed following acetate enrichment (wastewater-fed MFCs – CE = 22.0 ± 6.2%; acetate-enriched MFCs, fed wastewater – CE =58.5 ± 3.5%; Synthetic wastewater fed MFCs – CE = 22.0 ± 3.2%; acetate-enriched synthetic wastewater-fed MFCs – 28.7 ± 4.2%.) The relative abundance of Geobacter ssp. and CE represents the average of the nine replicate reactors inoculated with three different inocula for each substrate. Efforts to improve the performance of anodic microbial communities in MFCs utilizing complex organic substrates should therefore focus on enhancing the activity of organisms driving hydrolysis and fermentation rather the terminal-oxidizing electrogens.
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影响因子：
48
作者：
Callahan BJ;McMurdie PJ;Rosen MJ;Han AW;Johnson AJ;Holmes SP
通讯作者：
Holmes SP
影响因子：
5
作者：
Jung, Sokhee;Regan, John M.
通讯作者：
Regan, John M.
影响因子：
10.7
作者：
Kumar, Sudhir;Stecher, Glen;Tamura, Koichiro
通讯作者：
Tamura, Koichiro
影响因子：
3.3
作者：
Larsen, Steffen;Nielsen, Lars Peter;Schramm, Andreas
通讯作者：
Schramm, Andreas
DOI：
10.1099/00207713-48-2-469
发表时间：
1998-04-01
期刊：
INTERNATIONAL JOURNAL OF SYSTEMATIC BACTERIOLOGY
影响因子：
--
作者：
Lien, T;Madsen, M;Gjerdevik, K
通讯作者：
Gjerdevik, K