Effects of Cations on the Behaviour of Lipid Cubic Phases.
Effects of Cations on the Behaviour of Lipid Cubic Phases.
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DOI：
10.1038/s41598-017-08438-4
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发表时间：
2017-08-15
影响因子：
4.6
通讯作者：
Seddon A
中科院分区：
文献类型：
--
作者：
Brasnett C;Longstaff G;Compton L;Seddon A
由脂类形成的反双连续立方结构已经被证明在广泛的应用中，从用于结晶的蛋白质的主体基质，到用于纳米结构的模板。最近的工作集中在调整它们的性质以实现这种应用，通常是通过引入其他具有不同性质的脂类来操纵结构，如电荷或填充。然而，它们通常是在含有盐的溶液中制备的，抵消了带电的脂类等影响，并从根本上改变了所获得的结构。在这里，我们展示了双连续结构中静电溶胀之间的微妙相互作用，这些结构是由单油酯(MO)掺杂带负电荷的二油基磷脂酰甘油(DOPG)和两性离子二油基磷脂酰乙醇胺(DOPE)，加上单价和二价盐形成的。在带电相中加入盐的效果将结构从原始的立方相()改变为双钻石相()，同时仍允许晶格参数略有增加，最高可达一纳米。相比之下，在非带电相中加入盐对晶格参数的影响很小，但现在观察到在比纯水中更高的摩尔分数的掺杂下，发生了从()到反六方相(HII)的转变。
Inverse bicontinuous cubic structures formed by lipids have been demonstrated in a wide variety of applications, from a host matrix for proteins for crystallisation, to templates for nanoscale structures. Recent work has focused on tuning their properties to realize such applications, often by manipulating the structure by introducing other lipids with different properties such as charge or packing. However, they are often prepared in the presence of solutions containing salt, counteracting the effects, for example, charged lipids, and fundamentally changing the structures obtained. Here, we demonstrate the delicate interplay between electrostatic swelling in bicontinuous structures formed by monoolein (MO) doped with both negatively charged dioleyl phosphatidylglycerol (DOPG), and zwitterionic dioleyl phosphatidylethanolamine (DOPE), with the addition of mono- and divalent salts. The effect of adding salt to the charged phase changes the structure from the primitive cubic () to the double diamond phase () whilst still allowing for modest increases in lattice parameter of up to a nanometer. Contrasting this, the addition of salts to the non-charged phase, has minimal effect on the lattice parameter but now the transition from the () to the inverse hexagonal phase (HII) is observed occurring at higher mole fractions of DOPE than in pure water.
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