Assessment of Demineralization Inhibition Effects of Dentin Desensitizers Using Swept-Source Optical Coherence Tomography.
Assessment of Demineralization Inhibition Effects of Dentin Desensitizers Using Swept-Source Optical Coherence Tomography.
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DOI：
10.3390/ma14081876
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发表时间：
2021-04-09
期刊：
影响因子：
--
通讯作者：
Yoshiyama M
中科院分区：
文献类型：
--
作者：
Matsuzaki K;Shimada Y;Shinno Y;Ono S;Yamaji K;Ohara N;Sadr A;Sumi Y;Tagami J;Yoshiyama M
本研究的目的是通过体外预脱矿牙本质过敏症模型评价两种脱敏剂对牙本质脱矿的作用机制及抑制作用。在这项研究中，我们证实了我们初步实验中的牙齿过敏模型可以通过将牙本质盘浸泡在pH值为5.0的醋酸溶液中三天来制备。制备脱矿3d的牙本质盘，用含氟铝硅酸钙玻璃(Nanosseal，NS)或氟硅酸锌玻璃(Caredyne Shield，CS)的脱敏剂中的一种脱敏，然后再脱矿3d。同时制备脱矿3天(DE3)和脱矿6天(DE6)的牙本质盘。采用扫描光源光学相干断层扫描仪(SS-OCT)对实验后的牙本质盘进行扫描，对标本的横断面图进行成像，并对SS-OCT信号进行评估。获得了SS-OCT在300、500和700µm深度范围内的信号强度分布，计算了信号的积分强度和信号衰减系数。用扫描电子显微镜和能量色散X射线能谱分析牙本质盘的形态差异和剩余化学元素。CS组和NS组SS-OCT图像差异无统计学意义。然而，CS组和NS组的SS-OCT信号谱显示出比DE6组更小的衰减系数和更大的积分信号强度。NS组脱矿3d后牙本质表面仍可见反应性沉积，而CS组牙本质小管内可见含锌残留物。因此，在本研究中，CS和NS组都显示出对额外三天脱矿的抑制作用。结果表明，SS-OCT信号分析可用于监测牙本质脱矿及脱敏剂对牙本质脱矿的抑制作用。
The purpose of this study was to evaluate the mechanism of action and the inhibiting effects of two types of desensitizers against dentin demineralization using pre-demineralized hypersensitivity tooth model in vitro. In this study, we confirmed that a hypersensitivity tooth model from our preliminary experiment could be prepared by immersing dentin discs in an acetic acid-based solution with pH 5.0 for three days. Dentin discs with three days of demineralization were prepared and applied by one of the desensitizers containing calcium fluoro-alumino-silicate glass (Nanoseal, NS) or fluoro-zinc-silicate glass (Caredyne Shield, CS), followed by an additional three days of demineralization. Dentin discs for three days of demineralization (de3) and six days of demineralization (de6) without the desensitizers were also prepared. The dentin discs after the experimental protocol were scanned using swept-source optical coherence tomography (SS-OCT) to image the cross-sectional (2D) view of the samples and evaluate the SS-OCT signal. The signal intensity profiles of SS-OCT from the region of interest of 300, 500, and 700 µm in depth were obtained to calculate the integrated signal intensity and signal attenuation coefficient. The morphological differences and remaining chemical elements of the dentin discs were also analyzed using scanning electron microscopy and energy-dispersive X-ray spectroscopy. SS-OCT images of CS and NS groups showed no obvious differences between the groups. However, SS-OCT signal profiles for both the CS and NS groups showed smaller attenuation coefficients and larger integrated signal intensities than those of the de6 group. Reactional deposits of the desensitizers even after the additional three days of demineralization were observed on the dentin surface in NS group, whereas remnants containing Zn were detected within the dentinal tubules in CS group. Consequently, both CS and NS groups showed inhibition effects against the additional three days of demineralization in this study. Our findings demonstrate that SS-OCT signal analysis can be used to monitor the dentin demineralization and inhibition effects of desensitizers against dentin demineralization in vitro.
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影响因子：
2.9
作者：
Manz, Andrea S.;Attin, Thomas;Sahrmann, Philipp
通讯作者：
Sahrmann, Philipp
影响因子：
2.9
作者：
ROTH, S;FREUND, I
通讯作者：
FREUND, I
影响因子：
3.7
作者：
Choi, An-Na;Jang, II-Seok;Park, Jeong-Kil
通讯作者：
Park, Jeong-Kil
影响因子：
5
作者：
Niu, Li-na;Zhang, Wei;Pashley, David H.;Breschi, Lorenzo;Mao, Jing;Chen, Ji-hua;Tay, Franklin R.
通讯作者：
Tay, Franklin R.
DOI：
10.1002/jbm.b.34446
发表时间：
2019-08-05
影响因子：
3.4
作者：
Lee, Ester M. R.;Borges, Roger;Marques, Marcia M.
通讯作者：
Marques, Marcia M.