Response of sunflower (Helianthus annuus L.) leaf surface defenses to exogenous methyl jasmonate.
Response of sunflower (Helianthus annuus L.) leaf surface defenses to exogenous methyl jasmonate.
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DOI：
10.1371/journal.pone.0037191
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Rieseberg LH
中科院分区：
文献类型：
--
作者：
Rowe HC;Ro DK;Rieseberg LH
向日葵是一种常见的向日葵，它产生一系列复杂的次生化合物，分泌到腺毛中，腺毛是花的叶表面和花药附属物上的特殊结构。这些毛状体分泌物的主要成分是倍半萜内酯(STL)，这是一种由菊科植物大量产生的多种化合物，被认为有助于植物抵御草食性疾病。我们用外源茉莉酸甲酯处理野生和栽培的黄花草，茉莉酸甲酯是一种植物激素，可以介导植物对昆虫、食草动物和某些类别的真菌病原体的防御。野生向日葵的叶片上产生的腺毛密度高于栽培向日葵。液质联用(LC-MS)分析结果表明，野生和栽培黄花鱼的表面化学成分基本相似，但在相对大小和所检测到的化合物比例上有所不同。尽管观察到对茉莉酸甲酯处理的一致转录反应，我们没有发现对栽培或野生向日葵的腺毛密度或LC-MS图谱有显著影响，野生向日葵在茉莉酸处理的植株中显示出总体STL产生和叶片腺毛密度下降的趋势。这些结果表明，腺毛和相关化合物可能作为结构性防御或需要更高水平的刺激来诱导，而不是观察到的对外源茉莉酸的转录反应。减少对驯化品系的国防投资与预测的增产选择所造成的权衡是一致的；未来的研究将集中在遗传资源的开发上，以明确测试腺毛的生态作用及其对植物生长和适合性的相关影响。
Helianthus annuus, the common sunflower, produces a complex array of secondary compounds that are secreted into glandular trichomes, specialized structures found on leaf surfaces and anther appendages of flowers. The primary components of these trichome secretions are sesquiterpene lactones (STL), a diverse class of compounds produced abundantly by the plant family Compositae and believed to contribute to plant defense against herbivory. We treated wild and cultivated H. annuus accessions with exogenous methyl jasmonate, a plant hormone that mediates plant defense against insect herbivores and certain classes of fungal pathogens. The wild sunflower produced a higher density of glandular trichomes on its leaves than the cultivar. Comparison of the profiles of glandular trichome extracts obtained by liquid chromatography–mass spectroscopy (LC-MS) showed that wild and cultivated H. annuus were qualitatively similar in surface chemistry, although differing in the relative size and proportion of various compounds detected. Despite observing consistent transcriptional responses to methyl jasmonate treatment, we detected no significant effect on glandular trichome density or LC-MS profile in cultivated or wild sunflower, with wild sunflower exhibiting a declining trend in overall STL production and foliar glandular trichome density of jasmonate-treated plants. These results suggest that glandular trichomes and associated compounds may act as constitutive defenses or require greater levels of stimulus for induction than the observed transcriptional responses to exogenous jasmonate. Reduced defense investment in domesticated lines is consistent with predicted tradeoffs caused by selection for increased yield; future research will focus on the development of genetic resources to explicitly test the ecological roles of glandular trichomes and associated effects on plant growth and fitness.
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影响因子：
3.9
作者：
Göpfert, JC;Heil, N;Spring, O
通讯作者：
Spring, O
影响因子：
64.8
作者：
Harter, AV;Gardner, KA;Rieseberg, LH
通讯作者：
Rieseberg, LH
影响因子：
9.4
作者：
Bjorkman, Christer;Dalin, Peter;Ahrne, Karin
通讯作者：
Ahrne, Karin
DOI：
10.1073/pnas.1016508108
发表时间：
2011-04-05
影响因子：
11.1
作者：
Kempel, Anne;Schaedler, Martin;van Kleunen, Mark
通讯作者：
van Kleunen, Mark
影响因子：
5.9
作者：
Karray-Bouraoui, Najoua;Rabhi, Mokded;Smaoui, Abderrazak
通讯作者：
Smaoui, Abderrazak