Tear proteomics of orbital decompression for disfiguring exophthalmos in inactive thyroid-associated ophthalmopathy.
Tear proteomics of orbital decompression for disfiguring exophthalmos in inactive thyroid-associated ophthalmopathy.
复制标题
眼眶减压术治疗非活动性甲状腺相关眼病畸形性突眼的泪液蛋白质组学
DOI：
10.3892/etm.2020.9383
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发表时间：
2020-12
影响因子：
2.7
通讯作者：
Wang W
中科院分区：
文献类型：
--
作者：
Jiang L;Rong A;Wei R;Diao J;Ding H;Wang W
甲状腺相关性眼病（TAO）泪液蛋白质组学研究的进展和成果，对探索TAO发病机制和寻找潜在的治疗靶点具有重要意义。然而，眼窝减压术治疗失活性眼突出症的泪液蛋白质组学研究尚未得到充分的研究。在本研究中，采用眶减压术修复失活动性TAO患者的毁容性突出眼。失活动性TAO患者眼眶减压前后均收集泪液。采用液相色谱-串联质谱法（LC-MS/MS）研究泪液蛋白质组学的变化。然后采用生物信息学分析方法分析LC-MS/MS鉴定的差异表达蛋白（DEPs）的功能。眶减压1个月后睑裂高度和突出眼面积明显恢复，接近健康眼球的正常水平。在LC-MS/MS鉴定的669个蛋白中，83个蛋白在非活动性TAO患者和健康对照者的术前和术后阶段发生了显著变化。据预测，dep参与了许多信号通路。生物信息学分析显示，与免疫系统、代谢、程序性细胞死亡、囊泡介导转运、神经元系统和细胞外基质组织相关的途径可能在失活动性TAO患者的眼眶减压中发挥重要作用。综上所述，这些结果为眼眶减压治疗非活动性TAO患者毁容性突出眼的机制提供了初步的认识。
The progress and achievements that have been made in tear proteomics in thyroid-associated ophthalmopathy (TAO) are critical for exploring the pathogenesis of TAO and investigating potential therapeutic targets. However, the tear proteomics of orbital decompression for disfiguring exophthalmos in inactive TAO have yet to be properly investigated. In the present study, orbital decompression was performed to repair disfiguring exophthalmos in patients with inactive TAO. Tears were collected before and after orbital decompression in patients with inactive TAO. Liquid chromatography with tandem mass spectrometry (LC-MS/MS) was performed to explore the changes in tear proteomics. Bioinformatics analyses were then employed to analyze the functions of the differentially expressed proteins (DEPs) identified by LC-MS/MS. The palpebral fissure height and exophthalmia area were significantly restored after 1 month of orbital decompression such that they approached the normal levels identified in healthy eyeballs. Among the 669 proteins identified by LC-MS/MS, 83 proteins were changed significantly between the preoperative and postoperative stages in inactive TAO patients and healthy control individuals. The DEPs were predicted to be involved in numerous signaling pathways. Bioinformatics analyses revealed that pathways associated with the immune system, metabolism, programmed cell death, vesicle-mediated transport, neuronal system and extracellular matrix organization may fulfill significant roles in orbital decompression in patients with inactive TAO. Taken together, these results provided a preliminary understanding of the mechanism of orbital decompression for disfiguring exophthalmos in inactive TAO patients.
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影响因子：
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作者：
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