Expression of glutamate and inhibitory amino acid vesicular transporters in the rodent auditory brainstem.
Expression of glutamate and inhibitory amino acid vesicular transporters in the rodent auditory brainstem.
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DOI：
10.1002/cne.22521
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发表时间：
2011-02-01
影响因子：
2.5
通讯作者：
Oliver, Douglas L.
中科院分区：
文献类型：
--
作者：
Ito, Tetsufumi;Bishop, Deborah C.;Oliver, Douglas L.
谷氨酸是听觉系统中主要的兴奋性神经递质，但谷氨酸能神经元群体与其突触末梢分布之间的关联一直很困难。不同亚型的谷氨酸能末梢采用三种囊泡谷氨酸转运蛋白（VGLUT）之一来装载突触囊泡。最近，VGLUT 1和VGLUT 2终端被发现有不同的组织模式在下丘，这表明有不同类型的脑干听觉系统的神经元的投射到下丘。为了积极识别VGLUT表达神经元以及抑制性神经元在听觉脑干，我们使用原位杂交来识别VGLUT 1，VGLUT 2和VIAAT（GABA能和甘氨酸能终端使用的囊泡抑制性氨基酸转运蛋白）的mRNA。在成年大鼠和小鼠的听觉脑干和丘脑中的多巴胺能和抑制性神经元的亚群中发现了相似的表达模式。在单个神经元中观察到四种基因表达模式。1)VGLUT 2单独表达是普遍的模式。2)VGLUT 1和VGLUT 2共表达见于大多数核团的散在神经元，但常见于内侧膝状体和耳蜗腹侧核。3)VGLUT 1仅在颗粒细胞中表达。4)VIAAT表达在大多数核中是常见的，但在一些占主导地位。这些数据表明，VGLUT 1/2和VIAAT基因的表达可以识别听觉神经元的不同子集。这可能有助于识别听觉回路中的不同组件。
Glutamate is the main excitatory neurotransmitter in the auditory system, but associations between glutamatergic neuronal populations and the distribution of their synaptic terminations have been difficult. Different subsets of glutamatergic terminals employ one of three vesicular glutamate transporters (VGLUT) to load synaptic vesicles. Recently, VGLUT1 and VGLUT2 terminals were found to have different patterns of organization in the inferior colliculus suggesting that there are different types of glutamatergic neurons in the brainstem auditory system with projections to the colliculus. To positively identify VGLUT-expressing neurons as well as inhibitory neurons in the auditory brainstem, we used in situ hybridization to identify the mRNA for VGLUT1, VGLUT2, and VIAAT (the vesicular inhibitory amino acid transporter used by GABAergic and glycinergic terminals). Similar expression patterns were found in subsets of glutamatergic and inhibitory neurons in the auditory brainstem and thalamus of adult rats and mice. Four patterns of gene expression were seen in individual neurons. 1) VGLUT2 expressed alone was the prevalent pattern. 2) VGLUT1 co-expressed with VGLUT2 was seen in scattered neurons in most nuclei but was common in the medial geniculate body and ventral cochlear nucleus. 3) VGLUT1 expressed alone was found only in granule cells. 4) VIAAT expression was common in most nuclei but dominated in some. These data show that the expression of the VGLUT1/2 and VIAAT genes can identify different subsets of auditory neurons. This may facilitate the identification of different components in auditory circuits.
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影响因子：
56.9
作者：
Fremeau, RT;Kam, K;Edwards, RH
通讯作者：
Edwards, RH
影响因子：
2.5
作者：
CLERICI, WJ;COLEMAN, JR
通讯作者：
COLEMAN, JR
影响因子：
2.5
作者：
ADAMS, JC
通讯作者：
ADAMS, JC
影响因子：
56.9
作者：
Bellocchio, EE;Reimer, RJ;Edwards, RH
通讯作者：
Edwards, RH
影响因子：
2.8
作者：
Chernock, ML;Larue, DT;Winer, JA
通讯作者：
Winer, JA