Fitness of transgenic mosquito Aedes aegypti males carrying a dominant lethal genetic system.
Fitness of transgenic mosquito Aedes aegypti males carrying a dominant lethal genetic system.
复制标题
转基因蚊子埃及埃及雄性的适应性，载有主要的致命遗传系统。
DOI：
10.1371/journal.pone.0062711
复制
发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Reiter P
中科院分区：
文献类型：
--
作者：
Massonnet-Bruneel B;Corre-Catelin N;Lacroix R;Lees RS;Hoang KP;Nimmo D;Alphey L;Reiter P
OX 513 A是一种埃及伊蚊转基因株，经工程改造携带一种显性、非性别特异性、迟效致死遗传系统，该系统在四环素存在下受到抑制。它被设计用于称为RIDL®（释放携带显性致死基因的昆虫）的不育昆虫（SIT）害虫控制系统，通过该系统，转基因雄性被释放到田间与野生雌性交配;在没有四环素的情况下，这种交配的后代将无法存活。我们在实验室中研究了OX 513 A的交配适合度。雄性OX 513 A在诱导野生型雌性进一步交配的不应性方面与洛克菲勒（ROCK）雄性一样有效，并且它们使多个雌性授精的能力没有降低。它们的交配成功率较低，但当每种品系的一只雄性与一只ROCK雌性关在笼子里时，产生的后代比野生型比较品系（ROCK）多。交配成功率和生育率的组10名男性与不同比例的RIDL到ROCK竞争5个ROCK女性是相似的，但中位数寿命的RIDL男性有点（18%）低。我们得出结论，在实验室条件下的健身OX 513 A男性携带四环素抑制致死基因是可比的野生型比较菌株的男性。
OX513A is a transgenic strain of Aedes aegypti engineered to carry a dominant, non-sex-specific, late-acting lethal genetic system that is repressed in the presence of tetracycline. It was designed for use in a sterile-insect (SIT) pest control system called RIDL® (Release of Insects carrying a Dominant Lethal gene) by which transgenic males are released in the field to mate with wild females; in the absence of tetracycline, the progeny from such matings will not survive. We investigated the mating fitness of OX513A in the laboratory. Male OX513A were as effective as Rockefeller (ROCK) males at inducing refractoriness to further mating in wild type females and there was no reduction in their ability to inseminate multiple females. They had a lower mating success but yielded more progeny than the wild-type comparator strain (ROCK) when one male of each strain was caged with a ROCK female. Mating success and fertility of groups of 10 males—with different ratios of RIDL to ROCK—competing for five ROCK females was similar, but the median longevity of RIDL males was somewhat (18%) lower. We conclude that the fitness under laboratory conditions of OX513A males carrying a tetracycline repressible lethal gene is comparable to that of males of the wild-type comparator strain.
登录
查看更多内容
影响因子：
3
作者：
Catteruccia F;Crisanti A;Wimmer EA
通讯作者：
Wimmer EA
影响因子：
46.9
作者：
Harris, Angela F.;Nimmo, Derric;Alphey, Luke
通讯作者：
Alphey, Luke
影响因子：
46.9
作者：
Gong, P;Epton, MJ;Alphey, L
通讯作者：
Alphey, L
DOI：
10.4269/ajtmh.2012.11-0225
发表时间：
2012-04-01
影响因子：
3.3
作者：
Helinski, Michelle E. H.;Valerio, Laura;Harrington, Laura C.
通讯作者：
Harrington, Laura C.
DOI：
10.1186/jbiol143
发表时间：
2009-05-01
期刊：
Journal of Biology (London)
影响因子：
--
作者：
Alphey, Luke
通讯作者：
Alphey, Luke