An Unexpectedly Broad Thermal and Salinity-Tolerant Estuarine Methanogen Community.
An Unexpectedly Broad Thermal and Salinity-Tolerant Estuarine Methanogen Community.
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DOI：
10.3390/microorganisms8101467
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发表时间：
2020-09-24
期刊：
影响因子：
4.5
通讯作者：
Gray ND
中科院分区：
文献类型：
--
作者：
Blake LI;Sherry A;Mejeha OK;Leary P;Coombs H;Stone W;Head IM;Gray ND
在来自温带、热带和低温环境的长期浓缩后，鉴定出中等嗜热性(Tmax，~55°C)产甲烷菌。然而，生长温度较高(TOPT≥60°C)的嗜热产甲烷菌仅在高温环境中有报道。一种基于微观宇宙的方法被用来测量温带河口沉积物在不同温度和盐度范围内的甲烷产生率和产甲烷菌群落结构。我们报告了在温带河口沉积物(原位温度4-5℃)中进行的短期培养(<；48小时)发现甲烷菌的最佳活性达到70°C。对于相应的中温营养组，选择30℃乙酸盐、氢气或甲醇进行富集物，而在60℃时，只观察到水生基因营养菌(甲烷热细菌属)。由于这些产甲烷菌在原位温度下不是已知的活性，我们得出结论，这些产甲烷菌从高温生境中持续扩散。通过覆盖一系列温度和盐度的富集物，研究了嗜热产甲烷菌的可能来源。这些富集物表明，河口沉积物中含有甲烷菌，涵盖了大多数养殖物种的全球活动范围。我们认为河口是研究微生物功能的诱人的汇和源环境。
Moderately thermophilic (Tmax, ~55 °C) methanogens are identified after extended enrichments from temperate, tropical and low-temperature environments. However, thermophilic methanogens with higher growth temperatures (Topt ≥ 60 °C) are only reported from high-temperature environments. A microcosm-based approach was used to measure the rate of methane production and methanogen community structure over a range of temperatures and salinities in sediment from a temperate estuary. We report short-term incubations (<48 h) revealing methanogens with optimal activity reaching 70 °C in a temperate estuary sediment (in situ temperature 4–5 °C). While 30 °C enrichments amended with acetate, H2 or methanol selected for corresponding mesophilic trophic groups, at 60 °C, only hydrogenotrophs (genus Methanothermobacter) were observed. Since these methanogens are not known to be active under in situ temperatures, we conclude constant dispersal from high temperature habitats. The likely provenance of the thermophilic methanogens was studied by enrichments covering a range of temperatures and salinities. These enrichments indicated that the estuarine sediment hosted methanogens encompassing the global activity envelope of most cultured species. We suggest that estuaries are fascinating sink and source environments for microbial function study.
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影响因子：
2.3
作者：
Ding, Xia;Yang, Wei-Jun;Lu, Zhen-Mei
通讯作者：
Lu, Zhen-Mei
影响因子：
5.2
作者：
Cicerone, R.;Oremland, R.
通讯作者：
Oremland, R.
影响因子：
8.8
作者：
Mejeha, Obioma K.;Head, Ian M.;Gray, Neil D.
通讯作者：
Gray, Neil D.
DOI：
10.1155/2013/582646
发表时间：
2013
期刊：
Archaea (Vancouver, B.C.)
影响因子：
--
作者：
Torres-Alvarado Mdel R;Fernández FJ;Ramírez Vives F;Varona-Cordero F
通讯作者：
Varona-Cordero F
影响因子：
9.7
作者：
Liu, Pengfei;Klose, Melanie;Conrad, Ralf
通讯作者：
Conrad, Ralf