Cadmium interferes with auxin physiology and lignification in poplar.
Cadmium interferes with auxin physiology and lignification in poplar.
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DOI：
10.1093/jxb/err384
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发表时间：
2012-02
影响因子：
6.9
通讯作者：
Polle A
中科院分区：
文献类型：
--
作者：
Elobeid M;Göbel C;Feussner I;Polle A
镉（Cd）是一种植物毒性重金属，导致快速生长减少。为了研究Cd对植物生长激素生长素代谢的影响，以表达外源GH3：：GUS报告基因的白杨（Populus×canescens）为材料，在水培条件下，研究Cd对生长素代谢的影响。生长，光合性能，木质化，过氧化物酶活性，生长素浓度和GUS染色进行了测定，以记录GH3酶的活性在茎尖，伸长区，木材的径向生长区，并在根。Cd诱导的生长抑制表现为组织特异性降低，其顺序为：根>木材>新梢伸长和叶起始，而Cd浓度增加的顺序为：叶<木材<根。镉几乎取消了GH3信号在茎尖，但引起强烈的增加，在维管系统的根，以及在木质部的薄壁细胞。这些变化伴随着木质素和过氧化物酶活性的增加和生长素浓度的降低。由于GH3酶去除生长素从活性池共轭和作为生长和防御之间的介质，我们的数据表明，镉胁迫引发的GH3活动，这反过来又耗尽生长素在木材中，从而分流的代谢，以增强木质素的形成增加。
Cadmium (Cd) is a phytotoxic heavy metal that causes rapid growth reduction. To investigate if Cd interferes with the metabolism of auxin, a major growth hormone in plants, poplars (Populus×canescens) expressing a heterologous GH3::GUS reporter gene were exposed to 50 μM Cd in hydroponic solutions. Growth, photosynthetic performance, lignification, peroxidase activity, auxin concentration, and GUS staining were determined in order to record the activities of GH3 enzymes in the stem apex, the elongation zone, wood in the zone of radial growth, and in roots. Cd-induced growth reductions were tissue-specific decreasing in the order: roots>wood>shoot elongation and leaf initiation, whereas Cd concentrations increased in the order: leaves<wood<roots. Cd almost abolished the GH3 signal in the stem apex but caused strong increases in the vascular system of roots as well as in parenchymatic cells in the xylem. These changes were accompanied by increases in lignin and peroxidase activities and decreases in auxin concentrations. Since GH3 enzymes remove auxin from the active pool by conjugation and act as mediators between growth and defence, our data suggest that Cd stress triggered increases in GH3 activities which, in turn, depleted auxin in wood and thereby shunted the metabolism to enhanced formation of lignin.
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影响因子：
11.6
作者：
LIU, ZB;ULMASOV, T;GUILFOYLE, TJ
通讯作者：
GUILFOYLE, TJ
影响因子：
2
作者：
Chaoui, A;El Ferjani, E
通讯作者：
El Ferjani, E
DOI：
10.1016/0143-1471(85)90108-4
发表时间：
1985-01-01
期刊：
ENVIRONMENTAL POLLUTION SERIES A-ECOLOGICAL AND BIOLOGICAL
影响因子：
--
作者：
GODBOLD, DL;HUTTERMANN, A
通讯作者：
HUTTERMANN, A
影响因子：
4.8
作者：
Park, Jung-Eun;Park, Ju-Young;Park, Chung-Mo
通讯作者：
Park, Chung-Mo
影响因子：
5.7
作者：
Radotic, K;Ducic, T;Mutavdzic, D
通讯作者：
Mutavdzic, D