Paper spray screening and liquid chromatography/mass spectrometry confirmation for medication adherence testing: A two-step process.
Paper spray screening and liquid chromatography/mass spectrometry confirmation for medication adherence testing: A two-step process.
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DOI：
10.1002/rcm.8553
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发表时间：
2019-08-14
期刊：
影响因子：
--
通讯作者：
Bailey MJ
中科院分区：
文献类型：
--
作者：
Costa C;Frampas C;Longman KA;Palitsin V;Ismail M;Sears P;Nilforooshan R;Bailey MJ
纸喷雾提供了一个快速的筛选测试，而无需样品制备。纸喷雾的不完全提取允许使用更稳健、选择性和灵敏度更高的技术（如液相色谱/质谱法（LC/MS））进行进一步检测。在这里，我们开发了一个两步过程，即纸喷雾和LC/MS，以（1）快速筛选大量样品和（2）确认任何有争议的结果。这证明了从指纹测试药物依从性的适用性。在纸喷雾分析后，使用LC/MS分析滥用药物样品。所有分析均使用Q Exactive™ Plus Orbitrap™质谱仪完成。这两步程序应用于从服用维持剂量的抗精神病药物奎替鲁的患者中收集的指纹。发现纸喷雾对两种滥用药物和代谢物的提取效率在15%至35%之间（取决于分析物）。对于短的采集时间，发现提取效率在重复之间的变化小于30%，使得能够通过LC/MS进行后续分析。然后将该两步过程应用于从服用抗精神病药物喹替鲁胺的两名患者收集的指纹，这说明了如何使用LC/MS解决纸喷雾的阴性筛选结果，我们首次展示了使用纸喷雾和LC/MS对同一样品的连续分析，以及从指纹中检测抗精神病药物。我们建议，该工作流程也可以应用于与纸喷雾兼容的任何类型的样品，并且在只有一个样品可用于分析的情况下将特别方便。
Paper spray offers a rapid screening test without the need for sample preparation. The incomplete extraction of paper spray allows for further testing using more robust, selective and sensitive techniques such as liquid chromatography/mass spectrometry (LC/MS). Here we develop a two‐step process of paper spray followed by LC/MS to (1) rapidly screen a large number of samples and (2) confirm any disputed results. This demonstrates the applicability for testing medication adherence from a fingerprint. Following paper spray analysis, drugs of abuse samples were analysed using LC/MS. All analyses were completed using a Q Exactive™ Plus Orbitrap™ mass spectrometer. This two‐step procedure was applied to fingerprints collected from patients on a maintained dose of the antipsychotic drug quetiapine. The extraction efficiency of paper spray for two drugs of abuse and metabolites was found to be between 15 and 35% (analyte dependent). For short acquisition times, the extraction efficiency was found to vary between replicates by less than 30%, enabling subsequent analysis by LC/MS. This two‐step process was then applied to fingerprints collected from two patients taking the antipsychotic drug quetiapine, which demonstrates how a negative screening result from paper spray can be resolved using LC/MS. We have shown for the first time the sequential analysis of the same sample using paper spray and LC/MS, as well as the detection of an antipsychotic drug from a fingerprint. We propose that this workflow may also be applied to any type of sample compatible with paper spray, and will be especially convenient where only one sample is available for analysis.
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影响因子：
2.9
作者：
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