Requirement of direct contact between chondrocytes and macrophages for the maturation of regenerative cartilage.
Requirement of direct contact between chondrocytes and macrophages for the maturation of regenerative cartilage.
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DOI：
10.1038/s41598-021-01437-6
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发表时间：
2021-11-18
影响因子：
4.6
通讯作者：
Hikita A
中科院分区：
文献类型：
--
作者：
Kanda K;Asawa Y;Inaki R;Fujihara Y;Hoshi K;Hikita A
由培养的软骨细胞制备的再生软骨通常在体外不成熟，移植后成熟。虽然许多因素，包括宿主细胞和体液因素，已被证明会影响体内软骨成熟，宿主和供体细胞之间的直接细胞-细胞接触的要求仍有待验证。在这项研究中，我们研究了通过细胞-细胞接触促进软骨成熟的宿主细胞。基于对移植软骨细胞的分析，我们检查了内皮细胞和巨噬细胞的贡献。使用可渗透组织液同时阻断宿主细胞的半封闭装置，我们选择性地移植软骨细胞和HUVEC或未处理的/M1极化/M2极化RAW 264.7细胞。结果，未处理的RAW 264.7细胞诱导软骨再生。此外，体外共培养试验表明软骨细胞和RAW264.7细胞之间由RNA介导的通讯，表明细胞外囊泡参与了这一过程。这些发现为建立体外软骨再生的方法提供了见解。
Regenerative cartilage prepared from cultured chondrocytes is generally immature in vitro and matures after transplantation. Although many factors, including host cells and humoral factors, have been shown to affect cartilage maturation in vivo, the requirement of direct cell–cell contact between host and donor cells remains to be verified. In this study, we examined the host cells that promote cartilage maturation via cell–cell contact. Based on analysis of the transplanted chondrocytes, we examined the contribution of endothelial cells and macrophages. Using a semiclosed device that is permeable to tissue fluids while blocking host cells, we selectively transplanted chondrocytes and HUVECs or untreated/M1-polarized/M2-polarized RAW264.7 cells. As a result, untreated RAW264.7 cells induced cartilage regeneration. Furthermore, an in vitro coculture assay indicated communication between chondrocytes and RAW264.7 cells mediated by RNA, suggesting the involvement of extracellular vesicles in this process. These findings provide insights for establishing a method of in vitro cartilage regeneration.
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影响因子：
7.3
作者：
Lee H;Abston E;Zhang D;Rai A;Jin Y
通讯作者：
Jin Y
影响因子：
5.4
作者：
Schlegel, Werner;Nuernberger, Sylvia;Marlovits, Stefan
通讯作者：
Marlovits, Stefan
影响因子：
4.1
作者：
Wu, Ling;Prins, Henk-Jan;Karperien, Marcel
通讯作者：
Karperien, Marcel
影响因子：
14
作者：
Fujihara, Yuko;Takato, Tsuyoshi;Hoshi, Kazuto
通讯作者：
Hoshi, Kazuto
DOI：
10.1038/nrd.2016.245
发表时间：
2017-03
期刊：
Nature reviews. Drug discovery
影响因子：
--
作者：
Shi Y;Inoue H;Wu JC;Yamanaka S
通讯作者：
Yamanaka S