Virome diversity analysis reveals novel enteroviruses and a human picobirnavirus in stool samples from African green monkeys with diarrhea
Virome diversity analysis reveals novel enteroviruses and a human picobirnavirus in stool samples from African green monkeys with diarrhea
复制标题
病毒组多样性分析揭示了腹泻非洲绿猴粪便样本中的新型肠道病毒和人类小核糖核酸病毒
DOI：
10.1016/j.meegid.2020.104279
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发表时间：
2020-03
期刊：
影响因子：
--
通讯作者：
Mao Panyong
中科院分区：
文献类型：
--
作者：
Li Wenjuan;Qiang Xin;Qin Si;Huang Yong;Hu Yan;Bai Bingke;Hou Jun;Gao Rong;Zhang Xianglilan;Mi Zhiqiang;Fan Hang;Ye Huahu;Tong Yigang;Mao Panyong
识别动物中的病毒很重要，因为人类的大多数传染病都是由人畜共患病毒引起的。非洲绿色猴是一种广泛应用于生物医学研究的非人灵长类动物模型。本研究从10只腹泻的非洲绿色猴粪便中提取总RNA。使用高通量测序来表征病毒组。PCR和桑格测序法测定全基因组序列。观察到了巨大的病毒多样性。优势病毒为肠道病毒和小RNA病毒。六个肠道病毒基因组和picobirnavirus RNA依赖的RNA聚合酶序列进行了表征。5种肠道病毒属于2种新的基因型，1种属于EV-A92。与人微小RNA病毒分离株GPBV 6C 2的RNA依赖性RNA聚合酶序列同源性最高（93.48%）。本研究有助于确定非洲绿色猴中潜在的人畜共患病毒。需要进一步研究以阐明其在动物和人类中的致病作用。
It is important to identify viruses in animals because most infectious diseases in humans are caused by viruses of zoonotic origin. African green monkey is a widely used non-human primate model in biomedical investigations. In this study, total RNAs were extracted from stool samples of 10 African green monkeys with diarrhea. High-throughput sequencing was used to characterize viromes. PCR and Sanger sequencing were used to determine the full genome sequences. Great viral diversity was observed. The dominant viruses were enteroviruses and picobirnaviruses. Six enterovirus genomes and a picobirnavirus RNA-dependent RNA polymerase sequence were characterized. Five enteroviruses belonged to two putative new genotypes of speciesEnterovirus J. One enterovirus belonged to EV-A92. The picobirnavirus RNA-dependent RNA polymerase sequence had the highest nucleotide similarity (93.48%) with human picobirnavirus isolate GPBV6C2. The present study helped to identify the potential zoonotic viruses in African green monkeys. Further investigations are required to elucidate their pathogenic roles in animals and humans.
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影响因子：
3.7
作者：
Mombo IM;Lukashev AN;Bleicker T;Brünink S;Berthet N;Maganga GD;Durand P;Arnathau C;Boundenga L;Ngoubangoye B;Boué V;Liégeois F;Ollomo B;Prugnolle F;Drexler JF;Drosten C;Renaud F;Rougeron V;Leroy E
通讯作者：
Leroy E
DOI：
10.1093/eurpub/ckad160.455
发表时间：
2023-10-24
期刊：
The European Journal of Public Health
影响因子：
--
作者：
通讯作者：
--
DOI：
10.1098/rstb.2010.0031
发表时间：
2010-08-27
期刊：
Philosophical transactions of the Royal Society of London. Series B, Biological sciences
影响因子：
--
作者：
Sharp, Paul M;Hahn, Beatrice H
通讯作者：
Hahn, Beatrice H
影响因子：
10.7
作者：
Katoh K;Standley DM
通讯作者：
Standley DM
影响因子：
10.7
作者：
Kumar, Sudhir;Stecher, Glen;Tamura, Koichiro
通讯作者：
Tamura, Koichiro