Comprehensive analysis of oxylipins in human plasma using reversed-phase liquid chromatography-triple quadrupole mass spectrometry with heatmap-assisted selection of transitions.
Comprehensive analysis of oxylipins in human plasma using reversed-phase liquid chromatography-triple quadrupole mass spectrometry with heatmap-assisted selection of transitions.
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DOI：
10.1007/s00216-018-1446-3
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发表时间：
2019-01
影响因子：
4.3
通讯作者：
Zhang Q
中科院分区：
文献类型：
--
作者：
Chen GY;Zhang Q
氧磷脂是一类脂质介质，是多种多不饱和脂肪酸的代谢产物，在调节全身炎症中具有重要作用。要阐明它们在病理条件下的作用，需要准确地量化它们在生物样本中的水平。我们改进了一种基于超高效液相-多反应监测-质谱仪(UPLC-MRM-MS)的工作流程，对LC流动相添加剂、色谱柱和梯度条件进行了优化，实现了对人血浆中131种内源性氧脂的全面和特异的定量。我们使用热图辅助策略来识别独特的转变，以提高分析的选择性，并优化固相萃取程序，以实现更好的分析物回收。该方法根据FDA的指南进行了验证。总体而言，在测试浓度下，94.4%和95.7%的分析物分别在可接受的准确度(80-120%)和精密度(CV<；15%)内。大多数分析物的线性关系良好，R2>；0.99。该方法还使用标准参考物质-SRM 1950冰冻人血浆进行了验证，以证明实验室间的兼容性。
Oxylipins, a subclass of lipid mediators, are metabolites of various polyunsaturated fatty acids with crucial functions in regulation of systemic inflammation. Elucidation of their roles in pathological conditions requires accurate quantification of their levels in biological samples. We refined an ultra-performance liquid chromatography-multiple reaction monitoring-mass spectrometry (UPLC-MRM-MS)-based workflow for comprehensive and specific quantification of 131 endogenous oxylipins in human plasma, in which we optimized LC mobile phase additives, column and gradient conditions. We employed heatmap-assisted strategy to identify unique transitions to improve the assay selectivity and optimized solid phase extraction procedures to achieve better analyte recovery. The method was validated according to FDA guidelines. Overall, 94.4% and 95.7% of analytes at tested concentrations were within acceptable accuracy (80–120%) and precision (CV<15%), respectively. Good linearity for most analytes was obtained with R2 > 0.99. The method was also validated using a standard reference material – SRM 1950 frozen human plasma to demonstrate inter-lab compatibility.
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DOI：
10.1016/j.jchromb.2012.11.004
发表时间：
2012-12-12
影响因子：
3
作者：
Shinde, Dhananjay D.;Kim, Kwon-Bok;Shin, Jae Gook
通讯作者：
Shin, Jae Gook
影响因子：
4.3
作者：
Ostermann, Annika I.;Willenberg, Ina;Schebb, Nils Helge
通讯作者：
Schebb, Nils Helge
影响因子：
6.5
作者：
Sun, Ye;Koh, Hiromi W. L.;van Dam, Rob M.
通讯作者：
van Dam, Rob M.
影响因子：
4.1
作者：
Dolan, JW
通讯作者：
Dolan, JW
DOI：
10.1038/nri3859
发表时间：
2015-08
期刊：
Nature reviews. Immunology
影响因子：
--
作者：
Dennis EA;Norris PC
通讯作者：
Norris PC