Retinal Neurodegeneration in db/db Mice at the Early Period of Diabetes.
Retinal Neurodegeneration in db/db Mice at the Early Period of Diabetes.
复制标题
糖尿病早期 db/db 小鼠的视网膜神经变性。
DOI：
10.1155/2015/757412
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发表时间：
2015
影响因子：
1.9
通讯作者：
Liu Q
中科院分区：
文献类型：
--
作者：
Yang Q;Xu Y;Xie P;Cheng H;Song Q;Su T;Yuan S;Liu Q
目的。描述自发性2型糖尿病小鼠模型（db/db小鼠）神经感觉视网膜的功能和病理改变。方法。采用视网膜电图（PERG）、眼底荧光素血管造影（FFA）和光学相干断层扫描（OCT）对不同年龄（12、16、20、24和28周）的db/db （BKS/ db−/−）小鼠和杂合子窝仔（对照组）进行检查。免疫组化评价神经炎症组织学标志物IBA-1和F4/80。此外，通过末端dUTP镍端标记（TUNEL）测量视网膜神经节细胞死亡水平。结果。与对照组相比，糖尿病db/db小鼠在20周内观察到PERG反应的显著改变和视网膜神经节细胞（RGCs）凋亡的增加。IBA-1和F4/80在小胶质细胞/巨噬细胞中的表达在24周内变得明显，从而支持PERG的发现。此外，oct也显示28周龄db/db小鼠鼻视网膜和背视网膜明显变薄，整个实验过程中，FFA未检测到明显的视网膜微血管改变。结论。db/db小鼠糖尿病视网膜发生神经退行性改变，主要发生在视网膜神经节细胞层和内核层。但db/db小鼠视网膜血管未见明显异常。
Purpose. To describe both the functional and pathological alternations in neurosensory retina in a murine model of spontaneous type 2 diabetes (db/db mouse). Methods. db/db (BKS/DB−/−) mice and heterozygous littermates (as control group) at various ages (12, 16, 20, 24, and 28 weeks) were inspected with pattern electroretinogram (PERG), fundus fluorescein angiography (FFA), and optical coherence tomography (OCT). Histological markers of neuroinflammation (IBA-1 and F4/80) were evaluated by immunohistochemistry. In addition, levels of retinal ganglion cell death were measured by terminal dUTP nick-end labeling (TUNEL). Results. Significant alternations of PERG responses and increased retinal ganglion cells (RGCs) apoptosis were observed in diabetic db/db mice for 20-week period when compared with control group. IBA-1 and F4/80 expression in microglia/macrophages became evidently for 24-week period, thus supporting the PERG findings. Furthermore, obvious thinning of nasal and dorsal retina in 28-week-old db/db mice was also revealed by OCT. No visible retinal microvascular changes were detected by FFA throughout the experiments on db/db mice. Conclusions. Diabetic retina underwent neurodegenerative changes in db/db mice, which happened at retinal ganglion cell layer and inner nuclear layer. But there was no obvious abnormality in retinal vasculature on db/db mice.
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影响因子：
--
作者：
Gardner, Thomas W.;Abcouwer, Steven F.;Barber, Alistair J.;Jackson, Gregory R.
通讯作者：
Jackson, Gregory R.
影响因子：
--
作者：
Kern, Timothy S.
通讯作者：
Kern, Timothy S.
影响因子：
4.4
作者：
Chou, Tsung-Han;Porciatti, Vittorio
通讯作者：
Porciatti, Vittorio
DOI：
10.1083/jcb.119.3.493
发表时间：
1992-11
期刊：
The Journal of cell biology
影响因子：
--
作者：
Gavrieli Y;Sherman Y;Ben-Sasson SA
通讯作者：
Ben-Sasson SA
影响因子：
3.7
作者：
Bogdanov P;Corraliza L;Villena JA;Carvalho AR;Garcia-Arumí J;Ramos D;Ruberte J;Simó R;Hernández C
通讯作者：
Hernández C