Distribution and intrinsic membrane properties of basal forebrain GABAergic and parvalbumin neurons in the mouse.
Distribution and intrinsic membrane properties of basal forebrain GABAergic and parvalbumin neurons in the mouse.
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DOI：
10.1002/cne.23290
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发表时间：
2013-04-15
影响因子：
2.5
通讯作者：
Brown, Ritchie E.
中科院分区：
文献类型：
--
作者：
McKenna, James T.;Yang, Chun;Franciosi, Serena;Winston, Stuart;Abarr, Kathleen K.;Rigby, Matthew S.;Yanagawa, Yuchio;McCarley, Robert W.;Brown, Ritchie E.
基底前脑（BF）强烈调节皮质激活、睡眠稳态和注意力。参与这些过程的许多BF神经元是gaba能神经元，包括含有钙结合蛋白小白蛋白（PV）的投射神经元亚群。然而，鉴定中的技术困难阻碍了GABA能神经元和GABA/PV+神经元在小鼠中的精确分布或确定其内在膜特性。我们使用GABAergic （GAD67-GFP敲入小鼠）或PV+ （PV- tomato小鼠）表达荧光蛋白的小鼠来研究这些神经元。GAD67-GFP小鼠GABA免疫组化染色证实GFP选择性标记BF GABA能神经元。GFP+神经元和纤维分布于整个BF，以大细胞视前区（MCPO）密度最高。免疫组化结果显示，BF中PV+神经元以大（> ~ 20 μm）或中等（15 ~ 20 μm） GFP+神经元为主。大多数中、大型BF GFP+神经元，包括那些从新皮层逆行标记的神经元，在体外都是快速放电和自发活性的。它们表现出明显的超极化激活的内向电流和阈下“小穗”，暗示了电耦合。PV- tomato小鼠的PV+神经元具有类似的特性，但动作电位明显变窄，最大放电频率更高。另一群较小的GFP+神经元具有类似纹状体投射神经元的特性。BF - GABA/PV+神经元的快速放电和电耦合，以及它们对皮层中间神经元和丘脑网状核的投射，表明对觉醒和快速眼动睡眠典型的新皮层快速节律有强烈的同步控制。
The basal forebrain (BF) strongly regulates cortical activation, sleep homeostasis, and attention. Many BF neurons involved in these processes are GABAergic, including a subpopulation of projection neurons containing the calcium-binding protein, parvalbumin (PV). However, technical difficulties in identification have prevented a precise mapping of the distribution of GABAergic and GABA/PV+ neurons in the mouse or a determination of their intrinsic membrane properties. Here we used mice expressing fluorescent proteins in GABAergic (GAD67-GFP knock-in mice) or PV+ neurons (PV-Tomato mice) to study these neurons. Immunohistochemical staining for GABA in GAD67-GFP mice confirmed that GFP selectively labeled BF GABAergic neurons. GFP+ neurons and fibers were distributed throughout the BF, with the highest density in the magnocellular preoptic area (MCPO). Immunohistochemistry for PV indicated that the majority of PV+ neurons in the BF were large (>20 μm) or medium-sized (15–20 μm) GFP+ neurons. Most medium and large-sized BF GFP+ neurons, including those retrogradely labeled from the neocortex, were fast-firing and spontaneously active in vitro. They exhibited prominent hyperpolarization-activated inward currents and subthreshold “spikelets,” suggestive of electrical coupling. PV+ neurons recorded in PV-Tomato mice had similar properties but had significantly narrower action potentials and a higher maximal firing frequency. Another population of smaller GFP+ neurons had properties similar to striatal projection neurons. The fast firing and electrical coupling of BF GABA/PV+ neurons, together with their projections to cortical interneurons and the thalamic reticular nucleus, suggest a strong and synchronous control of the neocortical fast rhythms typical of wakefulness and REM sleep.
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影响因子：
3.3
作者：
Arrigoni, E.;Chamberlin, N. L.;McCarley, R. W.
通讯作者：
McCarley, R. W.
影响因子：
3.3
作者：
Brown, R. E.;Winston, S.;McCarley, R. W.
通讯作者：
McCarley, R. W.
影响因子：
2.5
作者：
BICKFORD, ME;GUNLUK, AE;SHERMAN, SM
通讯作者：
SHERMAN, SM
影响因子：
3.3
作者：
BRASHEAR, HR;ZABORSZKY, L;HEIMER, L
通讯作者：
HEIMER, L
影响因子：
3.4
作者：
Cape, EG;Jones, BE
通讯作者：
Jones, BE