Transcriptome profiling of chemosensory appendages in the malaria vector Anopheles gambiae reveals tissue- and sex-specific signatures of odor coding.
Transcriptome profiling of chemosensory appendages in the malaria vector Anopheles gambiae reveals tissue- and sex-specific signatures of odor coding.
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DOI：
10.1186/1471-2164-12-271
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发表时间：
2011-05-27
期刊：
影响因子：
4.4
通讯作者：
Zwiebel LJ
中科院分区：
文献类型：
--
作者：
Pitts RJ;Rinker DC;Jones PL;Rokas A;Zwiebel LJ
化学感受信号转导指导许多昆虫的行为，包括冈比亚按蚊，撒哈拉以南非洲地区人类疟疾的主要媒介。为了更好地理解蚊子化学感受的分子基础，我们使用全转录组RNA测序（RNA-seq）来比较成年雌性和雄性An的两个主要化学感受组织（触角和上颌须）之间的转录物表达谱。冈比亚。我们比较了每个性别的化学感受组织转录组和全身转录组，以确定化学感受增强基因。在分析的六个数据集中，我们检测到几乎所有已知的化学感受基因的表达，并发现它们在男性和女性的嗅觉组织中高度富集。虽然男性和女性的下颚须表现出严格的化学感受基因表达重叠，我们观察到男性和女性的触角之间的感官专业化的急性差异。雌性触角中化学感受基因的相对高表达水平揭示了其作为主要分配给化学感受的器官的作用。值得注意的是，这些基因的表达在雄性触角中高度保守，但相对水平要低得多。或者，与交配中的作用一致，雄性触角显示出听觉相关基因的显着增强，而雌性触角则观察到这些基因的增强，但程度较低。这些研究结果表明，化学感受谱，所定义的基因表达谱，在很大程度上是相似的女性和男性安。冈比亚。然而，假设感觉受体的表达水平与敏感性在每种情况下，我们认为，男性和女性的触角感知相同的刺激，但具有相反的接收优先级和敏感性。在这里，我们已经证明了使用RNA-seq来表征一种重要疾病载体的感觉特化，并为未来研究化学感觉过程奠定了基础。
Chemosensory signal transduction guides the behavior of many insects, including Anopheles gambiae, the major vector for human malaria in sub-Saharan Africa. To better understand the molecular basis of mosquito chemosensation we have used whole transcriptome RNA sequencing (RNA-seq) to compare transcript expression profiles between the two major chemosensory tissues, the antennae and maxillary palps, of adult female and male An. gambiae. We compared chemosensory tissue transcriptomes to whole body transcriptomes of each sex to identify chemosensory enhanced genes. In the six data sets analyzed, we detected expression of nearly all known chemosensory genes and found them to be highly enriched in both olfactory tissues of males and females. While the maxillary palps of both sexes demonstrated strict chemosensory gene expression overlap, we observed acute differences in sensory specialization between male and female antennae. The relatively high expression levels of chemosensory genes in the female antennae reveal its role as an organ predominately assigned to chemosensation. Remarkably, the expression of these genes was highly conserved in the male antennae, but at much lower relative levels. Alternatively, consistent with a role in mating, the male antennae displayed significant enhancement of genes involved in audition, while the female enhancement of these genes was observed, but to a lesser degree. These findings suggest that the chemoreceptive spectrum, as defined by gene expression profiles, is largely similar in female and male An. gambiae. However, assuming sensory receptor expression levels are correlated with sensitivity in each case, we posit that male and female antennae are perceptive to the same stimuli, but possess inverse receptive prioritizations and sensitivities. Here we have demonstrated the use of RNA-seq to characterize the sensory specializations of an important disease vector and grounded future studies investigating chemosensory processes.
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影响因子：
4.4
作者：
Das, Suchismita;Radtke, Andrea;Dimopoulos, George
通讯作者：
Dimopoulos, George
影响因子：
1.5
作者：
CHARLWOOD, JD;JONES, MDR
通讯作者：
JONES, MDR
影响因子：
4.4
作者：
Baton LA;Robertson A;Warr E;Strand MR;Dimopoulos G
通讯作者：
Dimopoulos G
DOI：
10.1073/pnas.88.12.5467
发表时间：
1991-06-01
影响因子：
11.1
作者：
AYER, RK;CARLSON, J
通讯作者：
CARLSON, J
DOI：
10.1523/jneurosci.6292-09.2010
发表时间：
2010-04-07
期刊：
The Journal of neuroscience : the official journal of the Society for Neuroscience
影响因子：
--
作者：
Bai L;Carlson JR
通讯作者：
Carlson JR