Genome-wide transcriptomic profiling of Anopheles gambiae hemocytes reveals pathogen-specific signatures upon bacterial challenge and Plasmodium berghei infection.
Genome-wide transcriptomic profiling of Anopheles gambiae hemocytes reveals pathogen-specific signatures upon bacterial challenge and Plasmodium berghei infection.
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DOI：
10.1186/1471-2164-10-257
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发表时间：
2009-06-05
期刊：
影响因子：
4.4
通讯作者：
Dimopoulos G
中科院分区：
文献类型：
--
作者：
Baton LA;Robertson A;Warr E;Strand MR;Dimopoulos G
冈比亚按蚊是人类疟疾的主要传播媒介。越来越多的证据表明，血细胞是蚊子对抗细菌和疟疾寄生虫的先天免疫反应的重要组成部分。为了进一步表征血细胞在蚊子免疫中的作用，我们对成年雌性安蚊进行了首次全基因组转录组分析。冈比亚红细胞感染后的两种细菌和疟疾寄生虫。我们鉴定了4047个在血细胞中表达的基因。冈比亚全基因组微阵列。相对于整个成年雌蚊，279个转录本在血细胞中显著富集，959个转录本表现出免疫攻击相关调控。血细胞对不同种类细菌和/或不同阶段疟疾寄生虫感染的全球转录组反应揭示了感染反应基因表达的离散、最小重叠、病原体特异性特征；其中105个代表推定的免疫相关基因，包括抗疟原虫因子。特别令人感兴趣的是，在疟疾寄生虫入侵蚊子中肠上皮期间，Imd和JNK免疫信号通路的各种成员的特定共同调节。我们对成年蚊子血细胞的全基因组转录组学分析揭示了基因调控的病原体特异性特征，并为未来的功能研究确定了几个新的候选基因。
The mosquito Anopheles gambiae is a major vector of human malaria. Increasing evidence indicates that blood cells (hemocytes) comprise an essential arm of the mosquito innate immune response against both bacteria and malaria parasites. To further characterize the role of hemocytes in mosquito immunity, we undertook the first genome-wide transcriptomic analyses of adult female An. gambiae hemocytes following infection by two species of bacteria and a malaria parasite. We identified 4047 genes expressed in hemocytes, using An. gambiae genome-wide microarrays. While 279 transcripts were significantly enriched in hemocytes relative to whole adult female mosquitoes, 959 transcripts exhibited immune challenge-related regulation. The global transcriptomic responses of hemocytes to challenge with different species of bacteria and/or different stages of malaria parasite infection revealed discrete, minimally overlapping, pathogen-specific signatures of infection-responsive gene expression; 105 of these represented putative immunity-related genes including anti-Plasmodium factors. Of particular interest was the specific co-regulation of various members of the Imd and JNK immune signaling pathways during malaria parasite invasion of the mosquito midgut epithelium. Our genome-wide transcriptomic analysis of adult mosquito hemocytes reveals pathogen-specific signatures of gene regulation and identifies several novel candidate genes for future functional studies.
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影响因子：
9.8
作者：
Dong, Yuemei;Taylor, Harry E;Dimopoulos, George
通讯作者：
Dimopoulos, George
影响因子：
6.7
作者：
Dong Y;Aguilar R;Xi Z;Warr E;Mongin E;Dimopoulos G
通讯作者：
Dimopoulos G
影响因子：
11.8
作者：
Boutros, M;Agaisse, H;Perrimon, N
通讯作者：
Perrimon, N
影响因子：
56.9
作者：
Christophides, GK;Zdobnov, E;Kafatos, FC
通讯作者：
Kafatos, FC
DOI：
10.1073/pnas.070357597
发表时间：
2000-03-28
影响因子：
11.1
作者：
Basset, A;Khush, RS;Lemaitre, B
通讯作者：
Lemaitre, B