Working with Commercially Available Quantum Dots for Immunofluorescence on Tissue Sections.
Working with Commercially Available Quantum Dots for Immunofluorescence on Tissue Sections.
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DOI：
10.1371/journal.pone.0163856
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发表时间：
2016
期刊：
影响因子：
3.7
通讯作者：
Bellamy CO
中科院分区：
文献类型：
--
作者：
Prost S;Kishen RE;Kluth DC;Bellamy CO
量子点是半导体荧光纳米晶体，与更常用的有机荧光染料相比，其表现出优异的特性。多年来，量子点缀合的产品已经以多种形式可用于组织切片的荧光成像，商标名为Qdot®。与传统的有机荧光染料相比，它们具有更高的亮度、窄的发射光谱、大的斯托克斯位移和光稳定性，这些共同使它们成为苛刻要求的荧光团的选择。Vivid Qdot是原始Qdot的最新替代品，经修饰以提高亮度，然而这影响了免疫组织化学常用条件下的荧光稳定性。我们在这里提出我们的调查的稳定性的原始和生动的量子点在溶液中和免疫组化，突出潜在的陷阱，并提出了一个协议，稳定和可靠的多重染色与目前市售的原始和生动的量子点。
Quantum dots are semiconductor fluorescent nanocrystals that exhibit excellent characteristics compared with more commonly used organic fluorescent dyes. For many years quantum dot conjugated products have been available in multiple forms for fluorescence imaging of tissue sections under the trademark name Qdot®. They have much increased brightness, narrow emission spectrum, large Stokes shift and photostability compared with conventional organic fluorescent dyes, which together make them the fluorophores of choice for demanding requirements. Vivid Qdots are recent replacements for original Qdots, modified to improve brightness, however this has affected the fluorescence stability in commonly used conditions for immunohistochemistry. We present here our investigation of the stability of original and Vivid Qdots in solution and in immunohistochemistry, highlight the potential pitfalls and propose a protocol for stable and reliable multiplex staining with current commercially available original and Vivid Qdots.
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影响因子：
16.6
作者：
Lim SJ;Zahid MU;Le P;Ma L;Entenberg D;Harney AS;Condeelis J;Smith AM
通讯作者：
Smith AM
影响因子：
3.2
作者：
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通讯作者：
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影响因子：
3.7
作者：
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通讯作者：
Bellamy, Christopher O. C.
DOI：
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发表时间：
2015-12-05
影响因子：
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作者：
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通讯作者：
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影响因子：
14
作者：
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通讯作者：
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