Recycling of cooking oil fume condensate for the production of rhamnolipids by Pseudomonas aeruginosa WB505
Recycling of cooking oil fume condensate for the production of rhamnolipids by Pseudomonas aeruginosa WB505
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回收食用油烟冷凝液用于铜绿假单胞菌WB505生产鼠李糖脂
DOI：
10.1007/s00449-019-02081-1
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发表时间：
2019-02
影响因子：
3.8
通讯作者：
Zhan Xiaobei
中科院分区：
文献类型：
--
作者：
Wu Jianrong;Zhang Jingbo;Zhang Hongtao;Gao Minjie;Liu Liming;Zhan Xiaobei
鼠李糖脂(Rhamnolipids，RLS)是一种极具应用潜力的阴离子生物表面活性剂。这项研究探索了从抽油烟机收集的烹饪油烟冷凝物(COFC)中生产RLS的可能性。获得了一株铜绿假单胞菌AB93066突变株，并将其用于以COFCs为底物生产RLS。在7-L发酵罐上发酵的菌体产量达到12.3g/L，MALDI-TOF MS分析表明Rha2-C10-C10和Rha-C10-C100是最丰富的菌体成分(分别占39.6%和26.4%)。临界胶束浓度为45.0 mg/L，水的表面张力从60.5mN/m降至25.3mN/m，以6种常见碳氢化合物为指标，得到的RLS的乳化系数均在60%以上，其中对苯的乳化系数为80.3%。COFC为RL生产提供了一种廉价的替代底物，而且合成过程相对无害和经济。
Rhamnolipids (RLs) are anionic biosurfactants with great application potential. This study explored the possibility of producing RLs from cooking oil fume condensates (COFCs) collected from range hoods. A mutant of Pseudomonas aeruginosa AB93066 was obtained and used to produce RLs from COFCs as a substrate. RL yields in a 7-L fermenter reached 12.3 g/L, and MALDI-TOF MS showed that Rha2-C10-C10and Rha-C10-C10are the most abundant (39.6% and 26.4%, respectively) RL components. The critical micellar concentration (CMC) of the RLs was 45.0 mg/L and the surface tension of water decreased from 60.5 to 25.3 mN/m. Using six kinds of common hydrocarbons as indices, the emulsification coefficients of the RLs obtained were found to exceed 60%; in particular, the emulsification coefficient for benzene was 80.3%. COFCs provide an inexpensive alternative as a substrate for RL production, and the synthetic process is relatively harmless and economical.
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影响因子：
3
作者：
Rebello, Sharrel;Asok, Aju K.;Jisha, M. S.
通讯作者：
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影响因子：
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作者：
Perfumo, Amedea;Rudden, Michelle;Banat, Ibrahim M.
通讯作者：
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影响因子：
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基于 pH 阶段控制的补料分批发酵工艺提高铜绿假单胞菌的鼠李糖脂产量
影响因子：
11.4
作者：
Zhu, Lingqing;Yang, Xue;Lu, Wenyu
通讯作者：
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影响因子：
5.5
作者：
Benincasa, M;Contiero, J;Moraes, IO
通讯作者：
Moraes, IO