Cloning and analysis of the HMG domains of ten Sox genes from Bombina maxima (Amphibia: Anura)
Cloning and analysis of the HMG domains of ten Sox genes from Bombina maxima (Amphibia: Anura)
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巨型蜂蚕（两栖类：无尾蜂）十个 Sox 基因 HMG 结构域的克隆和分析
DOI：
10.4314/ajb.v8i8.60145
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发表时间：
2009
影响因子：
--
通讯作者：
Nie Liuwang
中科院分区：
文献类型：
--
作者：
Wang Ning;Wang Jingjing;Nie Liuwang
Sox是一个大的基因家族，编码Sry相关的转录因子，并含有一个负责序列特异性DNA结合的HMG盒。根据人类SRY基因的保守基序（HMG-box）设计高度简并引物，从雄性和雌性大蹼铃蟾（Bombina maxima）中分别获得了10个含HMG-box的Sox基因克隆（BmSox 1a，BmSox 1b，BmSox 3a，BmSox 3b，BmSox 3c，BmSox 11 a，BmSox 11b，BmSox 11 c，BmSox 14，BmSox 33）。我们希望揭示这种蟾蜍中Sox基因的基因复制过程和基因树。序列分析表明，Sox 1、Sox 3和Sox 11基因可能存在重复。克隆的Sox基因与人SOX基因同源性较高。基于氨基酸序列的相似性，进行了系统发育分析，结果表明，10个HMG结构域编码序列中的9个是SoxB和SoxC组的成员。BmSox 33及其同源基因xSox 33（Xenopus laevis）仅在两栖类的盘舌科和奇足科中发现，而Sox 33在其他脊椎动物中尚未发现，Sox 33基因的起源是研究昆虫发生的一个有趣靶点。
Sox is a large gene family which encodes Sry-related transcription factors and contains a HMG box that is responsible for the sequence-specific DNA binding. In this paper, we obtained ten clones representing HMG box-containing Sox genes (BmSox1a, BmSox1b, BmSox3a, BmSox3b, BmSox3c, BmSox11a, BmSox11b, BmSox11c, BmSox14, BmSox33) from male and female Bombina maxima respectively and no sexual differences were observed, using highly degenerate primers designed from the conservative motif (HMG-box) of the human SRY gene. We want to reveal the gene duplication process and gene trees for the Sox genes in this toad. The sequences analysis indicated that Sox1, Sox3 and Sox11 gene may be duplicated. The cloned Sox genes shared high sequence identity to the homologous human SOX genes. Based on the amino acid sequence similarities, the phylogenetic analysis was carried out and the results suggested that nine of ten HMG domain-encoding sequences are members of the SoxB and SoxC groups. BmSox33 and its homologous gene xSox33(Xenopus laevis) were found only in Discoglossidae and Xenopodidae in amphibian, but Sox33 has not been found in other vertebrates up to now, and the origin of Sox33 gene is an interesting target in phylogenesis.
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影响因子：
3.9
作者：
Soullier, S;Jay, P;Laudet, V
通讯作者：
Laudet, V
影响因子：
4
作者：
C. Lobe;A. Nagy
通讯作者：
C. Lobe;A. Nagy
DOI：
10.1016/j.bbrc.2006.10.040
发表时间：
2006-12-08
影响因子：
3.1
作者：
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通讯作者：
Asashima, Makoto
影响因子：
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作者：
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影响因子：
2.4
作者：
Jifang Zheng;Mu-yuan Zhu
通讯作者：
Jifang Zheng;Mu-yuan Zhu