Surface micropattern limits bacterial contamination.
Surface micropattern limits bacterial contamination.
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DOI：
10.1186/2047-2994-3-28
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发表时间：
2014
影响因子：
5.5
通讯作者：
Reddy ST
中科院分区：
文献类型：
--
作者：
Mann EE;Manna D;Mettetal MR;May RM;Dannemiller EM;Chung KK;Brennan AB;Reddy ST
细菌表面污染导致医院感染的传播。用来控制表面污染的化学清洁剂往往有毒，而且使用不当。额外的无毒策略应与定期清洁相结合，以减少人为错误的风险，并进一步降低医院感染率。鲨鱼微图案(MP)的灵感来自鲨鱼皮，是一种有效的工具，可以减少表面的细菌负荷，而不添加有毒添加剂。本文研究了MP表面与光滑对照表面相比对减少甲氧西林敏感金黄色葡萄球菌(MSSA)和耐甲氧西林金黄色葡萄球菌(MRSA)定植的能力。比较了在丙烯酸薄膜中生产的MP和光滑表面在接种后的细菌污染和定植情况。在通过浸泡、喷雾和/或触摸方法接种后，对表面进行直接采样。最终，开发了一种组合试验来评估触摸转移接种结合干燥(持久性)后的细菌污染，以模拟诊所或医院环境中常见的环境污染场景。然后，结合转移和持久性试验，在MP之外测试抗菌铜降低MSSA和MRSA挑战的能力。与光滑的对照表面相比，MP减少了细菌污染，对数减少了87%-99%(LR = 0.90-2.18；p <； 0.05)。在减少金黄色葡萄球菌(MSSA和MRSA)表面污染方面，MP比99.9%纯铜合金C11000通过细菌的转移和持续作用更有效。与光滑对照相比，MP降低了97%(LR = 1.54；p <； 0.01)和94%(LR = 1.26；p <； 0.005)。抗菌铜对耐甲氧西林金黄色葡萄球菌污染无显著影响，但减少了80%的耐甲氧西林金黄色葡萄球菌污染(LR = 0.70；p <； 0.005)。本研究中开发的分析方法模拟医院环境污染事件，以展示MP在多种条件下限制污染的性能。抗菌铜已经应用于病房研究，以评估其对医院感染的影响，并显示HAI率下降。MP的类似实施有可能减少HAIS的发生率，尽管未来的临床研究将有必要验证MP的真正影响。
Bacterial surface contamination contributes to transmission of nosocomial infections. Chemical cleansers used to control surface contamination are often toxic and incorrectly implemented. Additional non-toxic strategies should be combined with regular cleanings to mitigate risks of human error and further decrease rates of nosocomial infections. The Sharklet micropattern (MP), inspired by shark skin, is an effective tool for reducing bacterial load on surfaces without toxic additives. The studies presented here were carried out to investigate the MP surfaces capability to reduce colonization of methicillin-sensitive Staphylococcus aureus (MSSA) and methicillin-resistant S. aureus (MRSA) compared to smooth control surfaces. The MP and smooth surfaces produced in acrylic film were compared for remaining bacterial contamination and colonization following inoculation. Direct sampling of surfaces was carried out after inoculation by immersion, spray, and/or touch methods. Ultimately, a combination assay was developed to assess bacterial contamination after touch transfer inoculation combined with drying (persistence) to mimic common environmental contamination scenarios in the clinic or hospital environment. The combination transfer and persistence assay was then used to test antimicrobial copper beside the MP for the ability to reduce MSSA and MRSA challenge. The MP reduced bacterial contamination with log reductions ranging from 87-99% (LR = 0.90-2.18; p < 0.05) compared to smooth control surfaces. The MP was more effective than the 99.9% pure copper alloy C11000 at reducing surface contamination of S. aureus (MSSA and MRSA) through transfer and persistence of bacteria. The MP reduced MSSA by as much as 97% (LR = 1.54; p < 0.01) and MRSA by as much as 94% (LR = 1.26; p < 0.005) compared to smooth controls. Antimicrobial copper had no significant effect on MSSA contamination, but reduced MRSA contamination by 80% (LR = 0.70; p < 0.005). The assays developed in this study mimic hospital environmental contamination events to demonstrate the performance of a MP to limit contamination under multiple conditions. Antimicrobial copper has been implemented in hospital room studies to evaluate its impact on nosocomial infections and a decrease in HAI rate was shown. Similar implementation of the MP has potential to reduce the incidence of HAIs although future clinical studies will be necessary to validate the MP’s true impact.
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影响因子：
2.7
作者：
Carman, ML;Estes, TG;Brennan, AB
通讯作者：
Brennan, AB
影响因子：
2.1
作者：
Chung, Kenneth K.;Schumacher, James F.;Brennana, Anthony B.
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影响因子：
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Weinstein, Robert A.
影响因子：
4.2
作者：
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