Runx3 transcription factor regulates ovarian functions and ovulation in female mice.
Runx3 transcription factor regulates ovarian functions and ovulation in female mice.
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DOI：
10.1262/jrd.2016-005
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发表时间：
2016-10-18
期刊：
影响因子：
--
通讯作者：
Takahashi S
中科院分区：
文献类型：
--
作者：
Ojima F;Saito Y;Tsuchiya Y;Kayo D;Taniuchi S;Ogoshi M;Fukamachi H;Takeuchi S;Takahashi S
我们先前证明Runx 3转录因子在小鼠的下丘脑、垂体和卵巢中表达，并且Runx 3敲除（Runx 3-/-）小鼠是无排卵的，它们的子宫萎缩。Runx 3 mRNA在卵泡颗粒细胞、前腹侧室周核（AVPV）和弓状核（ARC）中均有表达。在本研究中，我们研究了Runx 3基因敲除对类固醇生成相关酶基因表达的影响。我们发现，与8周龄野生型（wt）小鼠卵巢相比，Runx 3 −/−小鼠卵巢中Cyp 11 a1 mRNA表达降低。原位杂交分析表明，与野生型小鼠相比，Runx 3 −/−小鼠卵泡中表达Cyp 11 a1 mRNA的卵泡膜细胞的百分比降低。与Runx 3 −/−小鼠卵巢的改变雅阁，在Runx 3 −/−小鼠中，ARC中的Kiss 1 mRNA水平增加，而AVPV中的Kisspeptin mRNA水平降低，视前区的促性腺激素释放激素和卵泡刺激素β亚基基因增加。在Runx 3 −/−小鼠和野生型小鼠之间进行卵巢移植实验后，当Runx 3 −/−小鼠的卵巢移植到野生型小鼠时观察到黄体，但当野生型小鼠的卵巢移植到Runx 3 −/−小鼠时观察不到黄体，这表明下丘脑-垂体系统中的Runx 3可能驱动促性腺激素释放以诱导卵巢排卵。这些发现表明Runx 3在下丘脑-垂体-性腺轴中起着至关重要的作用。
We previously demonstrated that the Runx3 transcription factor is expressed in the hypothalami, pituitaries, and ovaries of mice, and that Runx3 knockout (Runx3−/−) mice are anovulatory and their uteri are atrophic. Runx3 mRNA expression was detected in the granulosa cells of ovarian follicles, and in the anteroventral periventricular nucleus (AVPV) and arcuate nucleus (ARC). In the present study, we examined the effects of Runx3 knockout on the gene expression of enzymes associated with steroidogenesis. We found decreased Cyp11a1 mRNA expression in Runx3−/− mouse ovaries compared with that in wild-type (wt) mouse ovaries at the age of 8 weeks. In situ hybridization analysis showed that the percentages of Cyp11a1 mRNA-expressing theca cells in follicles of Runx3−/− mice were decreased compared with those of wt mice. In accord with the alterations in Runx3−/− mouse ovaries, Kiss1 mRNA levels in ARC were increased, whereas mRNA levels of kisspeptin in AVPV were decreased, and gonadotropin-releasing hormone in the preoptic area and follicle-stimulating hormone β subunit gene were increased in Runx3−/− mice. Following an ovarian transplantation experiment between Runx3−/− mice and wt mice, corpora lutea were observed when ovaries from Runx3−/− mice were transplanted into wt mice, but not when those from wt mice were transplanted into Runx3−/− mice, suggesting that Runx3 in the hypothalamo-pituitary system may drive gonadotropin release to induce ovulation in the ovary. These findings indicate that Runx3 plays a crucial role in the hypothalamo-pituitary-gonadal axis.
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影响因子：
2.5
作者：
Sakuma, Atsuko;Fukamachi, Hiroshi;Takahashi, Sumio
通讯作者：
Takahashi, Sumio
影响因子：
6.7
作者：
Hoang, Yvonne D.;McTavish, Kirsten J.;Chang, R. Jeffrey;Shimasaki, Shunichi
通讯作者：
Shimasaki, Shunichi
影响因子：
4
作者：
Otto, F;Lübbert, M;Stock, M
通讯作者：
Stock, M
DOI：
10.1073/pnas.122066899
发表时间：
2002-06-11
影响因子：
11.1
作者：
Otsuka, F;Shimasaki, S
通讯作者：
Shimasaki, S
影响因子：
--
作者：
Jo, Misung;Curry, Thomas E., Jr.
通讯作者：
Curry, Thomas E., Jr.