Protective effects of polysaccharide from Artocarpus heterophyllus Lam. (jackfruit) pulp on non-alcoholic fatty liver disease in high-fat diet rats via PPAR and AMPK signaling pathways
Protective effects of polysaccharide from Artocarpus heterophyllus Lam. (jackfruit) pulp on non-alcoholic fatty liver disease in high-fat diet rats via PPAR and AMPK signaling pathways
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木菠萝多糖的保护作用。
DOI：
10.1016/j.jff.2022.105195
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发表时间：
2022-08
影响因子：
5.6
通讯作者：
Jun Cao
中科院分区：
文献类型：
--
作者：
Shunjiang Zeng;Yuzi Chen;Chenxi Wei;Lehe Tan;Chuan Li;Yanjun Zhang;Fei Xu;Kexue Zhu;Gang Wu;Jun Cao
·JFP-Ps降低高脂饮食大鼠的肝脏指数，改善肝功能。·JFP-Ps上调脂解基因(PPARα、Hs1、CPt1、Lpl)的表达。·JFP-Ps下调造脂基因和促炎细胞因子的表达。·JFP-Ps通过PPAR和AMPK途径减轻非酒精性脂肪性肝病。菠萝蜜多糖(JFP-Ps)具有多种生物活性。本研究旨在探讨JFP-Ps对高脂饮食诱导的大鼠非酒精性脂肪性肝病(NAFLD)的影响及其可能机制。苏木精-伊红染色和生化分析检测肝脏病理改变和肝功能。RT-qPCR和免疫印迹法检测脂质代谢相关蛋白和促炎性细胞因子的表达。结果表明，JFP-PS能降低高脂饮食大鼠的肝脏重量和肝脏指数，并影响其肝功能。此外，JFP-Ps还上调了脂解基因(PPARα、LPL、CPT1、HSL)的表达，下调了成脂基因(SREBP-1c、Acc、Fas)和促炎细胞因子(Tf-α、IL-6)的表达。JFP-Ps可增加p-AMPK的蛋白表达，降低SREBP-1c和AMPK的蛋白表达。提示JFP-Ps可能通过激活PPAR和AMPK信号通路改善肝功能，减轻NAFLD。
• JFP-Ps reduced liver index and improved liver function in high-fat diet rats. • JFP-Ps upregulated the expression of lipolytic genes (PPARα, HSL, CPT1, LPL). • JFP-Ps downregulated the lipogenic genes and the proinflammatory cytokines. • JFP-Ps alleviated non-alcoholic fatty liver disease via PPAR and AMPK pathways. A polysaccharide from jackfruit pulp (JFP-Ps) has been found to exhibit various types of bioactivities. This study aimed to investigate the effects and potential mechanisms of JFP-Ps on non-alcoholic fatty liver disease (NAFLD) induced by high-fat diet in rats. The pathological changes in the liver and liver function were determined using hematoxylin-eosin staining and biochemical analysis. RT-qPCR and Western blots were employed to study the expression of proteins associated with lipid metabolism and proinflammatory cytokines. Results showed that JFP-Ps reduced liver weight and liver index, and influenced liver function in high-fat diet rats. Furthermore, JFP-Ps up-regulated the lipolytic genes (PPARα, LPL, CPT1, HSL) expression, downregulated the expression of lipogenic genes (SREBP-1c, ACC, FAS) and proinflammatory cytokines (TNF-α, IL-6) genes. JFP-Ps increased the protein expression of p-AMPK, and decreased the protein expression of SREBP-1c and AMPK. These results revealed that JFP-Ps might improve liver function and alleviate NAFLD through activating PPAR and AMPK signaling pathways.
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