Sawhorse waveform voltammetry for selective detection of adenosine, ATP, and hydrogen peroxide.
Sawhorse waveform voltammetry for selective detection of adenosine, ATP, and hydrogen peroxide.
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DOI：
10.1021/ac501229c
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发表时间：
2014-08-05
影响因子：
7.4
通讯作者：
Venton, B. Jill
中科院分区：
文献类型：
--
作者：
Ross, Ashley E.;Venton, B. Jill
快速扫描循环伏安法（FSCV）是一种电化学技术，可以亚秒级检测体内神经递质。使用FSCV的腺苷检测已经变得越来越流行，但是由于在与腺苷相同或接近相同的电势下氧化的干扰剂，可能是困难的。三角形波形传统上用于FSCV，但已引入修改的波形以最大化分析物灵敏度并在高扫描速率下提供稳定性。在这里，一个修改的锯齿波形被用来最大限度地提高腺苷氧化的时间和操纵的形状的循环伏安图（CV）的分析物氧化在开关电位。优化波形包括以400 V/s的速度从−0.4 V扫描至1.35 V，并短暂保持1.0 ms，然后斜降至−0.4 V。该波形允许使用较低的开关电位进行腺苷检测。过氧化氢和ATP也在开关电位下氧化，并可能干扰体内腺苷测量;然而，它们的CV随锯马波形而改变，并且它们可以与腺苷区分开来。主成分分析（PCA）被用来确定锯齿波形是优于三角形波形之间的区别腺苷，过氧化氢，和ATP。在切片中，用PCA鉴定机械诱发的腺苷，并在POM-1操纵ATP代谢后观察ATP与腺苷比率的变化。锯齿波形是有用的腺苷，过氧化氢和ATP的歧视，并将促进这些分析物在体内更有信心的测量。
Fast-scan cyclic voltammetry (FSCV) is an electrochemistry technique which allows subsecond detection of neurotransmitters in vivo. Adenosine detection using FSCV has become increasingly popular but can be difficult because of interfering agents which oxidize at or near the same potential as adenosine. Triangle shaped waveforms are traditionally used for FSCV, but modified waveforms have been introduced to maximize analyte sensitivity and provide stability at high scan rates. Here, a modified sawhorse waveform was used to maximize the time for adenosine oxidation and to manipulate the shapes of cyclic voltammograms (CVs) of analytes which oxidize at the switching potential. The optimized waveform consists of scanning at 400 V/s from −0.4 to 1.35 V and holding briefly for 1.0 ms followed by a ramp back down to −0.4 V. This waveform allows the use of a lower switching potential for adenosine detection. Hydrogen peroxide and ATP also oxidize at the switching potential and can interfere with adenosine measurements in vivo; however, their CVs were altered with the sawhorse waveform and they could be distinguished from adenosine. Principal component analysis (PCA) was used to determine that the sawhorse waveform was better than the triangle waveform at discriminating between adenosine, hydrogen peroxide, and ATP. In slices, mechanically evoked adenosine was identified with PCA and changes in the ratio of ATP to adenosine were observed after manipulation of ATP metabolism by POM-1. The sawhorse waveform is useful for adenosine, hydrogen peroxide, and ATP discrimination and will facilitate more confident measurements of these analytes in vivo.
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影响因子：
5
作者：
Keithley, Richard B.;Wightman, R. Mark
通讯作者：
Wightman, R. Mark
影响因子：
4.2
作者：
Heien, MLAV;Phillips, PEM;Wightman, RM
通讯作者：
Wightman, RM
DOI：
10.1007/978-1-62703-251-3_16
发表时间：
2013-01-01
期刊：
Methods in molecular biology (Clifton, N.J.)
影响因子：
--
作者：
Roberts, James G;Lugo-Morales, Leyda Z;Sombers, Leslie A
通讯作者：
Sombers, Leslie A
影响因子：
1.9
作者：
Pedata, F;Gianfriddo, M;Melani, A
通讯作者：
Melani, A
影响因子：
7.4
作者：
Bath, BD;Michael, DJ;Wightman, RM
通讯作者：
Wightman, RM