Insights into the Mechanism of Bovine Spermiogenesis Based on Comparative Transcriptomic Studies.
Insights into the Mechanism of Bovine Spermiogenesis Based on Comparative Transcriptomic Studies.
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DOI：
10.3390/ani11010080
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发表时间：
2021-01-05
期刊：
影响因子：
--
通讯作者：
Wang D
中科院分区：
文献类型：
--
作者：
Li X;Duan C;Li R;Wang D
精子发生的任何不规则都会降低精液质量，并可能导致牛和人类的雄性不育。因此，我们研究了从圆形精子细胞到附睾精子的精子细胞差异转录组学，并将其与同期小鼠的转录组学进行比较。我们发现了与精子头部和尾部形成有关的差异表达基因（DEG），以及调控精子细胞遗传物质凝聚、变形和基因表达的表观遗传调控网络。根据不育报告中关于ART 3蛋白及其可能的表观遗传功能的研究，我们发现ART 3蛋白定位于精母细胞外，存在于圆形和细长的精子细胞中。有趣的是，我们观察到圆形和细长精子细胞上的ART 3蛋白定位于曲细精管的管腔附近。在附睾精子的头部和尾部也有分布，表明它在圆形精子细胞向精子的变形过程中起重要作用。我们的研究结果为牛精子发生的分子机制提供了新的见解，从而有助于提高牛的生殖潜力，并制定诊断和治疗男性不育症的策略。为了减少因精液质量低下而导致的生育力低下，为根除人类男性不育提供理论指导，我们对牛圆形、细长精子细胞和附睾精子的转录组进行了测序。以小鼠转录组为参照进行差异分析，并分析基因表达与小鼠的同源性趋势。首先，为了探讨影响精液质量的精子发生生理机制，比较了奶牛和小鼠精子发生过程中差异基因的同源性变化趋势。接下来，通过基因本体论（GO）、京都基因与基因组百科全书（KEGG）途径富集、蛋白质-蛋白质相互作用网络（PPI网络）和生物信息学分析，揭示了精子细胞从圆形向细长转变过程中顶体形成的调控网络。此外，在精子发生过程中，从细长的精子细胞到附睾精子，如泛素化，乙酰化，去乙酰化和糖基化，以及功能性ART 3基因的基因表达调控过程中可能发挥重要作用。因此，我们用免疫荧光分析法研究了它在生精小管和附睾精子中的定位，并对其结构和功能进行了预测。我们的研究结果提供了一个更深入的了解精子发生的过程，其中包括顶体形成，组蛋白替代，基因表达的精细调控。
Any irregularity in spermiogenesis reduces the quality of semen and may lead to male sterility in cattle and humans. Thus, we investigated the differential transcriptomics of spermatids from round spermatid to epididymal sperm and compared them with the transcriptomics of mice in the same period. We found differentially expressed genes (DEGs) involved in sperm head and tail formation, and epigenetic regulatory networks which regulated genetic material condensation, the deformation of the spermatid, and the expression of genes in it. According to the sterility report on the ART3 protein and its possible epigenetic function, we detected that it was localised outside the spermatocyte, in round and elongated spermatids. Interestingly, we observed that the ART3 protein on round and elongated spermatids was localised approximately to the lumen of seminiferous tubule. It was also localised on the head and tail part near the head in epididymal sperm, suggesting its important role in the deformation from round spermatids to sperm. Our findings provide new insights into the molecular mechanism underlying bovine spermiogenesis, thereby contributing to the improved reproductive potential of cattle and the development of strategies for the diagnosis and treatment of male infertility. To reduce subfertility caused by low semen quality and provide theoretical guidance for the eradication of human male infertility, we sequenced the bovine transcriptomes of round, elongated spermatids and epididymal sperms. The differential analysis was carried out with the reference of the mouse transcriptome, and the homology trends of gene expression to the mouse were also analysed. First, to explore the physiological mechanism of spermiogenesis that profoundly affects semen quality, homological trends of differential genes were compared during spermiogenesis in dairy cattle and mice. Next, Gene Ontology (GO), Kyoto Encyclopaedia of Genes and Genomes (KEGG) pathway enrichment, protein–protein interaction network (PPI network), and bioinformatics analyses were performed to uncover the regulation network of acrosome formation during the transition from round to elongated spermatids. In addition, processes that regulate gene expression during spermiogenesis from elongated spermatid to epididymal sperm, such as ubiquitination, acetylation, deacetylation, and glycosylation, and the functional ART3 gene may play important roles during spermiogenesis. Therefore, its localisation in the seminiferous tubules and epididymal sperm were investigated using immunofluorescent analysis, and its structure and function were also predicted. Our findings provide a deeper understanding of the process of spermiogenesis, which involves acrosome formation, histone replacement, and the fine regulation of gene expression.
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DOI：
10.1002/(sici)1098-2795(200006)56:2
发表时间：
2000-06-01
影响因子：
2.5
作者：
Balhorn, R;Brewer, L;Corzett, M
通讯作者：
Corzett, M
影响因子：
6.6
作者：
Hazzouri, M;Pivot-Pajot, C;Rousseaux, S
通讯作者：
Rousseaux, S
影响因子：
2.9
作者：
Friedrich, M;Grahnert, A;Hauschildt, S
通讯作者：
Hauschildt, S
影响因子：
3.6
作者：
Fair, S.;Lonergan, P.
通讯作者：
Lonergan, P.
影响因子：
5.7
作者：
Gill-Sharma MK;Choudhuri J;Ansari MA;D'Souza S
通讯作者：
D'Souza S