Inter-cellular forces orchestrate contact inhibition of locomotion.
Inter-cellular forces orchestrate contact inhibition of locomotion.
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DOI：
10.1016/j.cell.2015.02.015
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发表时间：
2015-04-09
期刊：
影响因子：
64.5
通讯作者：
Stramer BM
中科院分区：
文献类型：
--
作者：
Davis JR;Luchici A;Mosis F;Thackery J;Salazar JA;Mao Y;Dunn GA;Betz T;Miodownik M;Stramer BM
运动的接触抑制（CIL）是一个多方面的过程，导致许多细胞类型在碰撞时相互排斥。在发育过程中，这种看似不协调的反应是胚胎组织内细胞分散的关键驱动力。在这里，我们表明，果蝇血细胞需要一个精确的精心策划的CIL响应，他们的发展扩散。血细胞碰撞和随后的排斥涉及一个刻板的序列的运动学阶段，在细胞骨架动力学的全球变化调制。在体内追踪血细胞内的肌动蛋白逆行流动揭示了通过细胞间粘附的参与碰撞肌动蛋白网络的同步重组。这种细胞间的肌动蛋白离合器导致随后的层状张力的积累，触发了短暂的应力纤维的发展，这协调了细胞的排斥。我们的研究结果表明，在CIL流动肌动蛋白网络的物理耦合作为一个mechanotransducer，允许细胞触觉感知对方和协调他们的行为。胚胎分散血细胞中的CIL需要运动性的编排变化体内追踪肌动蛋白流动揭示了碰撞肌动蛋白网络的同步变化碰撞细胞之间的细胞间肌动蛋白离合器诱导板层张力张力的建立和释放协调了CIL反应果蝇巨噬细胞中运动的接触抑制涉及碰撞肌动蛋白网络的机械耦合。由此产生的板层张力的积累协调了反应并编排了细胞排斥。
Contact inhibition of locomotion (CIL) is a multifaceted process that causes many cell types to repel each other upon collision. During development, this seemingly uncoordinated reaction is a critical driver of cellular dispersion within embryonic tissues. Here, we show that Drosophila hemocytes require a precisely orchestrated CIL response for their developmental dispersal. Hemocyte collision and subsequent repulsion involves a stereotyped sequence of kinematic stages that are modulated by global changes in cytoskeletal dynamics. Tracking actin retrograde flow within hemocytes in vivo reveals synchronous reorganization of colliding actin networks through engagement of an inter-cellular adhesion. This inter-cellular actin-clutch leads to a subsequent build-up in lamellar tension, triggering the development of a transient stress fiber, which orchestrates cellular repulsion. Our findings reveal that the physical coupling of the flowing actin networks during CIL acts as a mechanotransducer, allowing cells to haptically sense each other and coordinate their behaviors. CIL in embryonic dispersing hemocytes requires choreographed changes in motility Tracking actin flow in vivo reveals synchronous changes in colliding actin networks An inter-cellular actin-clutch between colliding cells induces lamellar tension Tension buildup and release orchestrate the CIL response Contact inhibition of locomotion in Drosophila macrophages involves mechanical coupling of colliding actin networks. The resultant buildup in lamellar tension orchestrates the response and choreographs cell repulsion.
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影响因子：
7.8
作者：
Gardel, Margaret L.;Sabass, Benedikt;Ji, Lin;Danuser, Gaudenz;Schwarz, Ulrich S.;Waterman, Clare M.
通讯作者：
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影响因子：
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作者：
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影响因子：
21.3
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作者：
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通讯作者：
HEAYSMAN, JEM
DOI：
10.1073/pnas.95.8.4362
发表时间：
1998-04-14
影响因子：
11.1
作者：
Gloushankova, NA;Krendel, MF;Gelfand, IM
通讯作者：
Gelfand, IM