Field evaluation of poultry red mite (Dermanyssus gallinae) native and recombinant prototype vaccines.
Field evaluation of poultry red mite (Dermanyssus gallinae) native and recombinant prototype vaccines.
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DOI：
10.1016/j.vetpar.2017.06.020
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发表时间：
2017-09-15
影响因子：
2.6
通讯作者：
Nisbet AJ
中科院分区：
文献类型：
--
作者：
Bartley K;Turnbull F;Wright HW;Huntley JF;Palarea-Albaladejo J;Nath M;Nisbet AJ
天然和重组禽红螨疫苗的田间试验。接种可溶性螨提取物（SME）导致螨数量减少78%。抗体持久性差可能与缺乏重组鸡尾酒疫苗的效果有关。一种半保护性的自然获得性免疫可能会发展。疫苗接种是一种理想的新兴策略，以打击家禽红螨（PRM），鸡皮刺螨。我们在商业型笼舍设施中的产蛋鸡中进行试验，以测试可溶性螨提取物（SME）的天然制剂和含有免疫原性SME蛋白Deg-SRP-1、Deg-VIT-1和Deg-PUF-1的细菌表达形式的重组抗原混合物疫苗的疫苗效力。在12周龄和17周龄时，对母鸡（每组n = 384只）注射疫苗或仅注射佐剂（对照组），然后在10天后用PRM攻毒。监测PRM计数，在攻毒期结束时（攻毒后17周），与仅接种疫苗的对照组相比，含有接种SME的鸡的笼中的平均PRM计数降低了78%（p < 0.001）。当使用重组抗原混合疫苗重复试验时，在含有接种疫苗或仅接种疫苗的禽的笼中未观察到平均PRM数量的统计学显著差异。抗原特异性抗体水平和持续时间在提供疫苗诱导的和与疫苗相关的保护性免疫中的作用进行了讨论。
Field trial testing of a native and recombinant poultry red mite vaccines. Vaccination with a soluble mite extract (SME) resulted in a 78% reduction in mite numbers. Poor antibody persistence may relate to lack of effect of a recombinant cocktail vaccine. A semi-protective naturally acquired immunity may develop. Vaccination is a desirable emerging strategy to combat poultry red mite (PRM), Dermanyssus gallinae. We performed trials, in laying hens in a commercial-style cage facility, to test the vaccine efficacy of a native preparation of soluble mite extract (SME) and of a recombinant antigen cocktail vaccine containing bacterially-expressed versions of the immunogenic SME proteins Deg-SRP-1, Deg-VIT-1 and Deg-PUF-1. Hens (n = 384 per group) were injected with either vaccine or adjuvant only (control group) at 12 and 17 weeks of age and then challenged with PRM 10 days later. PRM counts were monitored and, at the termination of the challenge period (17 weeks post challenge), average PRM counts in cages containing birds vaccinated with SME were reduced by 78% (p < 0.001), compared with those in the adjuvant-only control group. When the trial was repeated using the recombinant antigen cocktail vaccine, no statistically significant differences in mean PRM numbers were observed in cages containing vaccinated or adjuvant-only immunised birds. The roles of antigen-specific antibody levels and duration in providing vaccine-induced and exposure-related protective immunity are discussed.
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影响因子：
4.4
作者：
Burgess, Stewart T. G.;Nunn, Francesca;Nisbet, Alasdair J.
通讯作者：
Nisbet, Alasdair J.
影响因子：
4.3
作者：
Jittapalapong, S.;Kaewhom, P.;Stich, R. W.
通讯作者：
Stich, R. W.
影响因子：
4.4
作者：
Kolotilin I;Topp E;Cox E;Devriendt B;Conrad U;Joensuu J;Stöger E;Warzecha H;McAllister T;Potter A;McLean MD;Hall JC;Menassa R
通讯作者：
Menassa R
影响因子：
4
作者：
Bartley K;Wright HW;Huntley JF;Manson ED;Inglis NF;McLean K;Nath M;Bartley Y;Nisbet AJ
通讯作者：
Nisbet AJ
影响因子：
4
作者：
Bartley, Kathryn;Nisbet, Alasdair J.;Huntley, John F.
通讯作者：
Huntley, John F.