Three-dimensional optic nerve head images using optical coherence tomography with a broad bandwidth, femtosecond, and mode-locked laser.
Three-dimensional optic nerve head images using optical coherence tomography with a broad bandwidth, femtosecond, and mode-locked laser.
复制标题
三维视神经头部图像使用光学相干断层扫描，带有宽带的带宽，飞秒和模式锁定激光。
DOI：
10.1007/s00417-014-2870-5
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发表时间：
2015-02
影响因子：
2.7
通讯作者：
Yoneya, Shin
中科院分区：
文献类型：
--
作者：
Shoji, Takuhei;Kuroda, Hiroto;Suzuki, Masayuki;Baba, Motoyoshi;Araie, Makoto;Yoneya, Shin
本研究的目的是使用宽波长、超高分辨率和光学相干断层扫描（OCT）系统，在体内展示视神经乳头（ONH）的精细层状结构。该高分辨率OCT系统基于200 nm带宽光谱仪和8飞秒超短锁模相干激光光源，能够实现轴向分辨率为2.0 μm的体内横截面ONH成像。共进行了300次视盘B超扫描，扫描像素为300 × 2048，扫描步长为10 μm。从这些图像中绘制三维图像，以获得视盘的n个面部图像。还对所有受试者进行了眼底照相、扫描激光检眼镜检查（SLO）和标准OCT检查。正常人36眼，青光眼患者10眼，平均年龄40.0 ± 10.0岁。以2.0 μm的步长重建了从ONH表面到更深层的连续表面图像。图像观察表明，在不同深度的椎板孔的形状和排列的变化。通过该技术在44只眼中识别出透明的椎板孔，与眼底照相机（在6只眼中识别出）、SLO（在14只眼中识别出）和标准OCT（在24只眼中识别出）相比（所有比较，p < 0.001）。ONH的精细结构可以使用我们的OCT在体内解析，提供改进的成像，可以用于研究和临床应用，以更好地表征与青光眼相关的解剖学和病理学特征。本文的在线版本（doi：10.1007/s 00417 -014-2870-5）包含补充材料，可供授权用户使用。
The aim of this study was to demonstrate the fine laminar structure of the optic nerve head (ONH), in vivo, using a broad wavelength, ultra-high resolution, and optically coherent tomography (OCT) system. This high-resolution OCT system, based on a 200 nm bandwidth spectrometer and an 8 femtosecond ultra-short, mode-locked, coherent laser light source, enabled in vivo cross-sectional ONH imaging with 2.0 μm axial resolution. A total of 300 optic disc B-scans, which consisted of 300 × 2048 pixels, were obtained in 10 μm steps. Three-dimensional images were rendered from these images to obtain n face images of the optic disc. Fundus photography, scanning laser ophthalmoscopy (SLO), and standard OCT were also performed for all subjects. Thirty-six eyes of normal subjects and ten eyes of glaucoma patients with mean age of 40.0 ± 10.0 years were enrolled in this study. Sequential en face images, from the ONH surface to deeper layers, were reconstructed in 2.0 μm steps. Observation of the images indicated variations in the shape and arrangement of the lamina pores at different depths. Clear lamina pores were identified by this technique in 44 eyes, compared with the fundus camera (identified in six eyes), SLO (identified in 14 eyes), and standard OCT (identified in 24 eyes) (all comparisons, p < 0.001). The fine structure of the ONH could be resolved in vivo using our OCT, providing improved imaging that can be used in research and clinical applications for a better characterization of the anatomical and pathological features associated with glaucoma. The online version of this article (doi:10.1007/s00417-014-2870-5) contains supplementary material, which is available to authorized users.
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影响因子：
2
作者：
Fontana, L;Bhandari, A;Hitchings, RA
通讯作者：
Hitchings, RA
影响因子：
13.7
作者：
Wojtkowski, M;Srinivasan, V;Duker, JS
通讯作者：
Duker, JS
影响因子：
13.7
作者：
Park, Sung Chul;De Moraes, Carlos Gustavo V.;Ritch, Robert
通讯作者：
Ritch, Robert
影响因子：
--
作者：
QUIGLEY, HA;ADDICKS, EM;MAUMENEE, AE
通讯作者：
MAUMENEE, AE
影响因子：
2.4
作者：
Maeda, H;Nakamura, M;Yamamoto, M
通讯作者：
Yamamoto, M