Catalase-coupled gold nanoparticles: comparison between the carbodiimide and biotin-streptavidin methods.
Catalase-coupled gold nanoparticles: comparison between the carbodiimide and biotin-streptavidin methods.
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DOI：
10.1016/j.actbio.2011.01.003
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发表时间：
2011-07
影响因子：
9.7
通讯作者：
Hilt, J. Zach
中科院分区：
文献类型：
--
作者：
Chirra, Hariharasudhan D.;Sexton, Travis;Biswal, Dipti;Hersh, Louis B.;Hilt, J. Zach
将蛋白质用于治疗应用需要蛋白质在体内治疗期间保持足够的活性。许多蛋白质在体内表现出短的半衰期，因此需要递送系统将它们用作治疗剂。相对的生物相容性和通过简单的化学形成功能化生物缀合物的能力使得金纳米颗粒成为蛋白质递送系统的优秀候选者。本文中，比较了用于将蛋白质偶联至金纳米颗粒的两种方案。在第一种方法中，生物素和链霉亲和素之间的强生物分子结合用于将过氧化氢酶偶联到金纳米颗粒的表面。在第二个方案中，使用碳二亚胺化学在羧酸涂覆的金纳米颗粒和过氧化氢酶的游离表面胺之间形成酰胺键。在这些协议中涉及的不同步骤的稳定性和动力学进行了研究，使用紫外-可见光谱，动态光散射，和透射电子显微镜。巯基十一烷酸与（N-（6-（生物素酰胺基）己基）-3′-（2′-吡啶基二硫代）丙酰胺的结合增加了生物素化金纳米颗粒的稳定性。尽管基于碳二亚胺化学的生物缀合方法表现出过氧化氢酶活性的降低，但是与巯基十一烷酸稳定的生物素化的金纳米颗粒相比，基于碳二亚胺化学的生物缀合方法导致每个金纳米颗粒更有活性的过氧化氢酶。两种偶联方案都导致负载有活性过氧化氢酶的金纳米颗粒。因此，这些金纳米颗粒系统和耦合协议代表了应用金纳米颗粒进行蛋白质递送的有前途的方法。
The use of proteins for therapeutic applications requires the protein to maintain sufficient activity for the period of in vivo treatment. Many proteins exhibit a short half-life in vivo and, thus, require delivery systems for them to be applied as therapeutics. The relative biocompatibility and the ability to form functionalized bioconjugates via simple chemistry make gold nanoparticles excellent candidates as protein delivery systems. Herein, two protocols for coupling proteins to gold nanoparticles were compared. In the first, the strong biomolecular binding between biotin and streptavidin was used to couple catalase to the surface of gold nanoparticles. In the second protocol, the formation of an amide bond between carboxylic acid coated gold nanoparticles and free surface amines of catalase using carbodiimide chemistry was performed. The stability and kinetics of the different steps involved in these protocols were studied using UV-Visible spectroscopy, dynamic light scattering, and transmission electron microscopy. The addition of mercaptoundecanoic acid in conjugation with (N-(6-(biotinamido)hexyl)-3′-(2′-pyridyldithio)-propionamide increased the stability of biotinylated gold nanoparticles. Although the carbodiimide chemistry based bioconjugation approach exhibited a decrease in catalase activity, the carbodiimide chemistry based bioconjugation approach resulted in more active catalase per gold nanoparticle compared to that of mercaptoundecanoic acid stabilized biotinylated gold nanoparticles. Both coupling protocols resulted in gold nanoparticles loaded with active catalase. Thus, these gold nanoparticle systems and coupling protocols represent promising methods for the application of gold nanoparticles for protein delivery.
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影响因子：
8.6
作者：
Phadtare, S;Kumar, A;Sastry, M
通讯作者：
Sastry, M
影响因子：
10.8
作者：
Eghtedari M;Liopo AV;Copland JA;Oraevsky AA;Motamedi M
通讯作者：
Motamedi M
影响因子：
7.4
作者：
Hirsch, LR;Jackson, JB;West, J
通讯作者：
West, J
影响因子：
5.5
作者：
RICHES, AC;SHARP, JG;SMITH, SV
通讯作者：
SMITH, SV
DOI：
10.1073/pnas.2232479100
发表时间：
2003-11-11
影响因子：
11.1
作者：
Hirsch, LR;Stafford, RJ;West, JL
通讯作者：
West, JL